Anti-PSD95抗体[7E3-1B8] (ab13552)


  • 产品名称Anti-PSD95抗体[7E3-1B8]
    参阅全部 PSD95 一抗
  • 描述
    小鼠单克隆抗体[7E3-1B8] to PSD95
  • 经测试应用适用于: WB, ICC/IF, IHC-P, IHC-Fr, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Rat, Cow, Human
  • 免疫原

    Recombinant full length protein (Rat).

  • 阳性对照
    • Rat and mouse brain tissue extract.



Our Abpromise guarantee covers the use of ab13552 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa.
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration. PubMed: 23209820
Flow Cyt Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.


  • 功能Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ASIC3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B.
  • 组织特异性Brain.
  • 序列相似性Belongs to the MAGUK family.
    Contains 1 guanylate kinase-like domain.
    Contains 3 PDZ (DHR) domains.
    Contains 1 SH3 domain.
  • 结构域The PDZ domain 3 mediates interaction with ADR1B.
    The L27 domain near the N-terminus of isoform 2 is required for HGS/HRS-dependent targeting to postsynaptic density.
  • 翻译后修饰Palmitoylation of isoform 1 is required for targeting to postsynaptic density.
  • 细胞定位Cell membrane. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell projection, axon. Cell junction, synapse. High levels in postsynaptic density of neurons in the forebrain. Also in presynaptic region of inhibitory synapses formed by cerebellar basket cells on axon hillocks of Purkinje cells.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Discs large homolog 4 antibody
    • Disks large homolog 4 antibody
    • DLG 4 antibody
    • Dlg4 antibody
    • DLG4_HUMAN antibody
    • FLJ97752 antibody
    • FLJ98574 antibody
    • Human post synaptic density protein 95 antibody
    • Post synaptic density protein 95 antibody
    • Postsynaptic density protein 95 antibody
    • PSD 95 antibody
    • PSD-95 antibody
    • PSD95 antibody
    • SAP 90 antibody
    • SAP-90 antibody
    • SAP90 antibody
    • Synapse associated protein 90 antibody
    • Synapse-associated protein 90 antibody
    • Tax interaction protein 15 antibody
    see all

Anti-PSD95 antibody [7E3-1B8] 图像

  • Western blot analysis of bovine brain tissue extract, probed with ab13552.
  • ab13552 at 1/100 dilution staining PSD95 in mouse backskin tissue section by Immunohistochemistry (Bouin's fixed paraffin embedded tissue sections). Tissue underwent heat mediated antigen retrieval in microwave with 2, 5 minutes incubation intervals in citrate buffer. The image show basal, hair follicle and dermal staining.

  • Central Panel: ab13552 staining PSD95 in rat primary hipoocampal neurons by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton and blocked with 3% BSA for 15 minutes at 20°C. Samples were incubated with primary antibody (1/200 in PBS-BSA 3%) for 30 minutes at 20°C. An Cy5®-conjugated Donkey polyclonal to mouse IgG (dilution 1/500) was used as secondary antibody.
    Left-hand panel: Homer GFP.
    Right-hand panel: Merge

    See Abreview

  • Overlay histogram showing K562 cells stained with ab13552 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13552, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
  • Immunohistochemical analysis of frozen mouse retinal sections, detecting photoreceptor synaptic endings by staining PSD95 (red) with ab13552.

    Sections were fixed in 4% paraformaldehyde before incubating with primary antibody (1/500). An AlexaFluor®568-conjugated anti-mouse IgG (1/1000) was used as the secondary antibody.

Anti-PSD95 antibody [7E3-1B8] (ab13552)参考文献

This product has been referenced in:
  • Gray EJ  et al. Src homology 2 domain containing protein 5 (SH2D5) binds the breakpoint cluster region protein, BCR, and regulates levels of Rac1-GTP. J Biol Chem 289:35397-408 (2014). Read more (PubMed: 25331951) »
  • Barone I  et al. Environmental enrichment extends photoreceptor survival and visual function in a mouse model of retinitis pigmentosa. PLoS One 7:e50726 (2012). IHC-Fr ; Mouse . Read more (PubMed: 23209820) »

See all 4 Publications for this product

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I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful. Before deciding how to proceed, I would like to investigate this particula...

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Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this lot of antibody.

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Thank you for confirming these details and for your cooperation.

I am sorry ab13552 did not perform well enough for your experiments and for the inconvenience this has caused. But I am happy that we have an alternative available that hopeful...

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Thank you for your answer.

Unfortunately, all the PSD-95 antibodies that we can guarantee for IHC-FoFr are rabbit polyclonals (ab16493, ab16495 and ab18258).

Having said this, we have goat polyclonal anti-PSD-95 antibody ab12093 a...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Primary hippocampal neurons)
Specification Primary hippocampal neurons
Fixative Paraformaldehyde
Permeabilization Yes - Triton 0.3%
Blocking step BSA as blocking agent for 15 minute(s) · Concentration: 3% · Temperature: 20°C

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提交于 Mar 11 2010