Anti-PSD95抗体[7E3-1B8] - Synaptic Marker (ab13552)
Key features and details
- Mouse monoclonal [7E3-1B8] to PSD95 - Synaptic Marker
- Suitable for: WB, ICC/IF, IHC-P, Flow Cyt
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgG1
概述
-
产品名称
Anti-PSD95抗体[7E3-1B8] - Synaptic Marker
参阅全部 PSD95 一抗 -
描述
小鼠单克隆抗体[7E3-1B8] to PSD95 - Synaptic Marker -
宿主
Mouse -
经测试应用
适用于: WB, ICC/IF, IHC-P, Flow Cytmore details -
种属反应性
与反应: Mouse, Rat, Cow, Human -
免疫原
Recombinant full length protein corresponding to Rat PSD95.
-
阳性对照
- ICC/IF: Rat primary hippocampal neurons; HaCaT cells; mouse backskin tissue section. WB: Rat brain membrane lysate; bovine brain tissue extract. Flow Cyt: K562 cells. IHC-P: Mouse backskin tissue.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
纯度
Protein G purified -
克隆
单克隆 -
克隆编号
7E3-1B8 -
同种型
IgG1 -
研究领域
相关产品
-
Compatible Secondaries
-
Conjugation kits
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab13552于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa.
|
|
ICC/IF | (1) |
Use at an assay dependent concentration.
|
IHC-P |
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
|
Flow Cyt |
Use 1µg for 106 cells.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
说明 |
---|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa. |
ICC/IF
Use at an assay dependent concentration. |
IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Flow Cyt
Use 1µg for 106 cells. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
靶标
-
功能
Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ASIC3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B. -
组织特异性
Brain. -
序列相似性
Belongs to the MAGUK family.
Contains 1 guanylate kinase-like domain.
Contains 3 PDZ (DHR) domains.
Contains 1 SH3 domain. -
结构域
The PDZ domain 3 mediates interaction with ADR1B.
The L27 domain near the N-terminus of isoform 2 is required for HGS/HRS-dependent targeting to postsynaptic density. -
翻译后修饰
Palmitoylation of isoform 1 is required for targeting to postsynaptic density. -
细胞定位
Cell membrane. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell projection, axon. Cell junction, synapse. High levels in postsynaptic density of neurons in the forebrain. Also in presynaptic region of inhibitory synapses formed by cerebellar basket cells on axon hillocks of Purkinje cells. - Information by UniProt
-
数据库链接
- Entrez Gene: 100137840 Cow
- Entrez Gene: 1742 Human
- Entrez Gene: 13385 Mouse
- Entrez Gene: 29495 Rat
- Omim: 602887 Human
- SwissProt: P78352 Human
- SwissProt: Q62108 Mouse
- SwissProt: P31016 Rat
see all -
别名
- Discs large homolog 4 antibody
- Disks large homolog 4 antibody
- DLG 4 antibody
see all
图片
-
Central Panel: ab13552 staining PSD95 in rat primary hippocampal neurons by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton and blocked with 3% BSA for 15 minutes at 20°C. Samples were incubated with primary antibody (1/200 in PBS-BSA 3%) for 30 minutes at 20°C. An Cy5®-conjugated Donkey polyclonal to mouse IgG (dilution 1/500) was used as secondary antibody.
Left-hand panel: Homer GFP.
Right-hand panel: Merge. -
Western blot analysis of bovine brain tissue extract, probed with ab13552. Western blot analysis of bovine brain tissue extract, probed with ab13552.
-
Overlay histogram showing K562 cells stained with ab13552 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13552, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback. -
ab13552 at 1/100 dilution staining PSD95 in mouse backskin tissue section by Immunohistochemistry (Bouin's fixed paraffin embedded tissue sections). Tissue underwent heat mediated antigen retrieval in microwave with 2, 5 minutes incubation intervals in citrate buffer. The image show basal, hair follicle and dermal staining.
-
Immunocytochemistry/ Immunofluorescence analysis of HaCaT cells labeling PSD95 with ab13552 at 1/100 dilution. Cells were fixed with 00% cold methanol for 10 minutes at -20°C. A FITC Goat Anti-Mouse antibody at 1/50 was used as secondary. The image shows Filamentouslike staining.
-
Anti-PSD95 antibody [7E3-1B8] - Synaptic Marker (ab13552) at 1/1000 dilution + Rat brain membrane lysate.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (47)
ab13552 被引用在 47 文献中.
- Fu Q et al. P-Rex2 mediation of synaptic plasticity contributes to bone cancer pain. Mol Pain 18:17448069221076460 (2022). PubMed: 35083941
- Milky B et al. Long-term adherence of human brain cells in vitro is enhanced by charged amine-based plasma polymer coatings. Stem Cell Reports 17:489-506 (2022). PubMed: 35180396
- Huang Q et al. Inhibition of ERK1/2 regulates cognitive function by decreasing expression levels of PSD-95 in the hippocampus of CIH rats. Eur J Neurosci 55:1471-1482 (2022). PubMed: 35243702
- Marcatti M et al. Aβ/tau oligomer interplay at human synapses supports shifting therapeutic targets for Alzheimer's disease. Cell Mol Life Sci 79:222 (2022). PubMed: 35377002
- Lu Q et al. Selective activation of ABCA1/ApoA1 signaling in the V1 by magnetoelectric stimulation ameliorates depression via regulation of synaptic plasticity. iScience 25:104201 (2022). PubMed: 35479414