Anti-PSD93抗体(ab12097)

概述

  • 产品名称Anti-PSD93抗体
    参阅全部 PSD93 一抗
  • 描述
    山羊多克隆抗体to PSD93
  • 经测试应用适用于: ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Rat, Human
  • 免疫原

    Synthetic peptide derived from residues 1 - 100 of Mouse PSD93.

    .

  • 阳性对照
    • This antibody gave a positive result when used in the following formaldehyde fixed cell lines: SKNSH
  • 常规说明


    PSD93 is part of the PSD-95/SAP90 family containing a modular structure with three PDZ-, one SH3- and a guanylate kinase-like domain. PSD93 is expressed in discrete neuronal populations as well as in specific non-neuronal cells. PSD93, like PSD-95, binds to NMDA receptors and to the neuronal nitric oxide synthase (NOS). PSD93 and PSD95 can heteromultimerize with each other and are recruited into the same NMDA receptor and K+ channel clusters. PSD93, however, is unique among PSD95/SAP90 family members in its expression in Purkinje neuron cell bodies and dendrites.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.025% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • Primary antibody说明PSD93 is part of the PSD-95/SAP90 family containing a modular structure with three PDZ-, one SH3- and a guanylate kinase-like domain. PSD93 is expressed in discrete neuronal populations as well as in specific non-neuronal cells. PSD93, like PSD-95, binds to NMDA receptors and to the neuronal nitric oxide synthase (NOS). PSD93 and PSD95 can heteromultimerize with each other and are recruited into the same NMDA receptor and K+ channel clusters. PSD93, however, is unique among PSD95/SAP90 family members in its expression in Purkinje neuron cell bodies and dendrites.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab12097 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 10 µg/ml.
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 0.3 - 3 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).

靶标

  • 功能Required for perception of chronic pain through NMDA receptor signaling. Regulates surface expression of NMDA receptors in dorsal horn neurons of the spinal cord. Interacts with the cytoplasmic tail of NMDA receptor subunits as well as inward rectifying potassium channels. Involved in regulation of synaptic stability at cholinergic synapses. Part of the postsynaptic protein scaffold of excitatory synapses.
  • 序列相似性Belongs to the MAGUK family.
    Contains 1 guanylate kinase-like domain.
    Contains 3 PDZ (DHR) domains.
    Contains 1 SH3 domain.
  • 结构域An N-terminally truncated L27 domain is predicted in isoform 2 at positions 1 through 27.
  • 翻译后修饰Palmitoylation of isoform 1 is not required for targeting to postsynaptic density.
  • 细胞定位Cell membrane. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell junction, synapse. Membrane. Cell projection, axon. Concentrated in soma and postsynaptic density of a subset of neurons.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Channel associated protein of synapse 110 antibody
    • Channel associated protein of synapses 110kD antibody
    • Channel-associated protein of synapse-110 antibody
    • Chapsyn 110 antibody
    • Chapsyn-110 antibody
    • Chapsyn110 antibody
    • Discs, large homolog 2 (Drosophila) antibody
    • Disks large homolog 2 antibody
    • DKFZp781D1854 antibody
    • DKFZp781E0954 antibody
    • Dlg 2 antibody
    • dlg2 antibody
    • DLG2_HUMAN antibody
    • Dlgh 2 antibody
    • Dlgh2 antibody
    • FLJ37266 antibody
    • Gm1197 antibody
    • MGC131811 antibody
    • Postsynaptic density protein PSD 93 antibody
    • Postsynaptic density protein PSD-93 antibody
    • Postsynaptic density protein PSD93 antibody
    • PSD 93 antibody
    • PSD93 antibody
    see all

Anti-PSD93 antibody 图像

  • ICC/IF image of ab12097 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab12097 at 10µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 donkey anti- goat (ab150133) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.



  • Performed under reducing conditions.

    Predicted band size : 110 kDa

    Chris Anderson, Wellcome Trust Sanger Institute, United Kingdom

    Western blot using mouse brain extract (10µg protein/lane) to test specificity of PSD93 (ab12097); PSD93 immunising peptide (ab12392) was used as a control.

    Lanes
    1. Ladder (kDa)
    2. 0.3µg/ml ab12097
    3. 1µg/ml ab12097
    4. 3µg/ml ab12097
    5. 3µg/ml ab12097 + 1µg/ml immunising peptide (ab12392)



  • Performed under reducing conditions.

    Predicted band size : 110 kDa


    Exposure time : 10 seconds

    This image is courtesy of Chris Anderson, Wellcome Trust Sanger Institute, United Kingdom

    Western blot using mouse brain extract (10µg protein/lane) to test specificity of PSD93 (ab12097); PSD93 immunising peptide (ab12392) was used as a control.

    Lanes
    1. Ladder (kDa)
    2. 0.3µg/ml ab12097
    3. 1µg/ml ab12097
    4. 3µg/ml ab12097
    5. 3µg/ml ab12097 + 1µg/ml immunising peptide (ab12392)

  • ab12097 PSD staining (10µg/ml) on mouse brain (5µm sagittal section on slide; 4% PFA perfused & paraffin embedded). Staining observed as expected in the hippocampus, frontal cortex and cortex with ab12097. Blue=haemotoxylin nuclear counter-stain.
  • ab12097 PSD staining (10µg/ml) on mouse brain (5µm sagittal section on slide; 4% PFA perfused & paraffin embedded). Granular staining observed as expected in [A] cerebellum and [B] hippocampus with ab12097. Blue=haemotoxylin nuclear counter-stain. Images taken with X10 objective.

Anti-PSD93 antibody (ab12097)参考文献

ab12097 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Sample Zebrafish Tissue sections (Retina, plexiform layers)
Specification Retina, plexiform layers
Permeabilization Yes - Triton X
Fixative Paraformaldehyde
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提交于 Sep 05 2014

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