Anti-psbA2抗体(ab65578)

概述

  • 产品名称
    Anti-psbA2抗体
  • 描述
    兔多克隆抗体to psbA2
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Arabidopsis thaliana, Tobacco
    预测可用于: Plants
  • 免疫原

    Synthetic peptide:

    PVDI DGIREPVAGS LLYGNNIISG AVVPS

    , corresponding to amino acids 57-85 of Synechocystis sp. PCC 6803 psbA2 (NP_439906.1). The peptide is conserved in D1 protein sequences from all photosynthetic organisms

  • 阳性对照
    • Arabidopsis and tobacco chloroplast, and Synechocystis thylakoid proteins.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.01% Sodium Azide
  • 纯度
    Whole antiserum
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab65578 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明
    WB: 1/1000. Detects a band of approximately 35 kDa (predicted molecular weight of precursor: 39 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性
      psbA2, also known as psbA2 photosystem II D1 protein, is one the major proteins of the reaction centre of photosystem II which carries the QB-binding site. D1 reduces the reaction centre P680* and provides the ligands to the manganese cluster of the water-oxidizing complex at the lumenal side of thylakoid membrane.
    • 细胞定位
      Cellular thylakoid membrane; Multi-pass membrane protein
    • 数据库链接
      • 别名
        • 32 kDa thylakoid membrane protein 2 antibody
        • Photosystem II protein D1 2 antibody
        • Photosystem Q(B) protein 2 antibody
        • psbA 2 antibody
        • psbA2 antibody
        • psbA2 photosystem II D1 protein antibody
        • psbA3 antibody
        see all

      Anti-psbA2 antibody 图像

      • All lanes : Anti-psbA2 antibody (ab65578) at 1/1000 dilution

        Lane 1 : Arabidopsis chloroplast proteins
        Lane 2 : Tobacco chloroplast proteins
        Lane 3 : Synechocystis thylakoid proteins

        Developed using the ECL technique

        Predicted band size : 39 kDa
        Observed band size : 35 kDa (why is the actual band size different from the predicted?)

      实验方案

      Anti-psbA2 antibody (ab65578)参考文献

      ab65578 has not yet been referenced specifically in any publications.

      Product Wall

      Application
      Western blot
      Loading amount
      25 µg
      Gel Running Conditions
      Reduced Denaturing (12% TGX Bio-Rad Gel)
      Sample
      Chlamydomonas reinhardtii Cell lysate - whole cell (C. reinhardtii lysate)
      Specification
      C. reinhardtii lysate
      Treatment
      2 M Urea for 1 hr on ice + loading buffer
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
      Username

      Javier Gimpel

      Verified customer

      提交于 Apr 04 2014

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

      注册