Anti-psbA2抗体(ab65578)

概述

  • 产品名称
    Anti-psbA2抗体
  • 描述
    兔多克隆抗体to psbA2
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Arabidopsis thaliana, Tobacco
    预测可用于: Plants
  • 免疫原

    Synthetic peptide:

    PVDI DGIREPVAGS LLYGNNIISG AVVPS

    , corresponding to amino acids 57-85 of Synechocystis sp. PCC 6803 psbA2 (NP_439906.1). The peptide is conserved in D1 protein sequences from all photosynthetic organisms

  • 阳性对照
    • Arabidopsis and tobacco chloroplast, and Synechocystis thylakoid proteins.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.01% Sodium Azide
  • 纯度
    Whole antiserum
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab65578 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明
    WB: 1/1000. Detects a band of approximately 35 kDa (predicted molecular weight of precursor: 39 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性
      psbA2, also known as psbA2 photosystem II D1 protein, is one the major proteins of the reaction centre of photosystem II which carries the QB-binding site. D1 reduces the reaction centre P680* and provides the ligands to the manganese cluster of the water-oxidizing complex at the lumenal side of thylakoid membrane.
    • 细胞定位
      Cellular thylakoid membrane; Multi-pass membrane protein
    • 数据库链接
      • 别名
        • 32 kDa thylakoid membrane protein 2 antibody
        • Photosystem II protein D1 2 antibody
        • Photosystem Q(B) protein 2 antibody
        • psbA 2 antibody
        • psbA2 antibody
        • psbA2 photosystem II D1 protein antibody
        • psbA3 antibody
        see all

      图片

      • All lanes : Anti-psbA2 antibody (ab65578) at 1/1000 dilution

        Lane 1 : Arabidopsis chloroplast proteins
        Lane 2 : Tobacco chloroplast proteins
        Lane 3 : Synechocystis thylakoid proteins

        Developed using the ECL technique

        Predicted band size : 39 kDa
        Observed band size : 35 kDa (why is the actual band size different from the predicted?)

      实验方案

      文献

      ab65578 has not yet been referenced specifically in any publications.

      客户评价及客户问答

      Application
      Western blot
      Loading amount
      25 µg
      Gel Running Conditions
      Reduced Denaturing (12% TGX Bio-Rad Gel)
      Sample
      Chlamydomonas reinhardtii Cell lysate - whole cell (C. reinhardtii lysate)
      Specification
      C. reinhardtii lysate
      Treatment
      2 M Urea for 1 hr on ice + loading buffer
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
      Username

      Javier Gimpel

      Verified customer

      提交于 Apr 04 2014

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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