概述

  • 产品名称Anti-PSAP抗体[PASE/4LJ]
    参阅全部 PSAP 一抗
  • 描述
    小鼠单克隆抗体[PASE/4LJ] to PSAP
  • 特异性This antibody does not inhibit the enzymatic activity of PSAP. It reacts with non-neoplastic adult and fetal prostatic glands, primary and metastatic prostatic carcinomas. It shows no cross reaction with other phosphatases.
  • 经测试应用适用于: IHC-P, ICC/IF, Flow Cytmore details
  • 种属反应性
    与反应: Human
    不与反应: Rat, Rabbit, Dog
  • 免疫原

    Purified PSAP from Human seminal plasma.

  • 阳性对照
    • Normal prostate or prostate carcinoma.

性能

应用

Our Abpromise guarantee covers the use of ab80717 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/1500.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use 0.1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能The lysosomal degradation of sphingolipids takes place by the sequential action of specific hydrolases. Some of these enzymes require specific low-molecular mass, non-enzymic proteins: the sphingolipids activator proteins (coproteins).
    Saposin-A and saposin-C stimulate the hydrolysis of glucosylceramide by beta-glucosylceramidase (EC 3.2.1.45) and galactosylceramide by beta-galactosylceramidase (EC 3.2.1.46). Saposin-C apparently acts by combining with the enzyme and acidic lipid to form an activated complex, rather than by solubilizing the substrate.
    Saposin-B stimulates the hydrolysis of galacto-cerebroside sulfate by arylsulfatase A (EC 3.1.6.8), GM1 gangliosides by beta-galactosidase (EC 3.2.1.23) and globotriaosylceramide by alpha-galactosidase A (EC 3.2.1.22). Saposin-B forms a solubilizing complex with the substrates of the sphingolipid hydrolases.
    Saposin-D is a specific sphingomyelin phosphodiesterase activator (EC 3.1.4.12).
  • 疾病相关Defects in PSAP are the cause of combined saposin deficiency (CSAPD) [MIM:611721]; also known as prosaposin deficiency. CSAPD is due to absence of all saposins, leading to a fatal storage disorder with hepatosplenomegaly and severe neurological involvement.
    Defects in PSAP saposin-B region are the cause of leukodystrophy metachromatic due to saposin-B deficiency (MLD-SAPB) [MIM:249900]. MLD-SAPB is an atypical form of metachromatic leukodystrophy. It is characterized by tissue accumulation of cerebroside-3-sulfate, demyelination, periventricular white matter abnormalities, peripheral neuropathy. Additional neurological features include dysarthria, ataxic gait, psychomotr regression, seizures, cognitive decline and spastic quadriparesis.
    Defects in PSAP saposin-C region are the cause of atypical Gaucher disease (AGD) [MIM:610539]. Affected individuals have marked glucosylceramide accumulation in the spleen without having a deficiency of glucosylceramide-beta glucosidase characteristic of classic Gaucher disease, a lysosomal storage disorder.
    Defects in PSAP saposin-A region are the cause of atypical Krabbe disease (AKRD) [MIM:611722]. AKRD is a disorder of galactosylceramide metabolism. AKRD features include progressive encephalopathy and abnormal myelination in the cerebral white matter resembling Krabbe disease.
    Note=Defects in PSAP saposin-D region are found in a variant of Tay-Sachs disease (GM2-gangliosidosis).
  • 序列相似性Contains 2 saposin A-type domains.
    Contains 4 saposin B-type domains.
  • 翻译后修饰This precursor is proteolytically processed to 4 small peptides, which are similar to each other and are sphingolipid hydrolase activator proteins.
    N-linked glycans show a high degree of microheterogeneity.
    The one residue extended Saposin-B-Val is only found in 5% of the chains.
  • 细胞定位Lysosome.
  • Information by UniProt
  • 数据库链接
  • 别名
    • A1 activator antibody
    • Cerebroside sulfate activator antibody
    • Co-beta-glucosidase antibody
    • Component C antibody
    • CSAct antibody
    • Dispersin antibody
    • GLBA antibody
    • Glucosylceramidase activator antibody
    • Proactivator polypeptide antibody
    • Proactivator polypeptide precursor antibody
    • Prosaposin (sphingolipid activator protein 1) antibody
    • prosaposin (variant Gaucher disease and variant metachromatic leukodystrophy) antibody
    • Prosaposin antibody
    • Protein A antibody
    • Protein C antibody
    • PSAP antibody
    • SAP-1 antibody
    • SAP-2 antibody
    • SAP_HUMAN antibody
    • SAP1 antibody
    • Saposin A antibody
    • Saposin B antibody
    • Saposin B Val antibody
    • Saposin C antibody
    • Saposin D antibody
    • Saposin-D antibody
    • Saposins antibody
    • Sgp1 antibody
    • Sphingolipid activator protein 1 antibody
    • Sphingolipid activator protein 2 antibody
    • Sulfated glycoprotein 1 antibody
    • Sulfatide/GM1 activator antibody
    see all

Anti-PSAP antibody [PASE/4LJ] 图像

  • Overlay histogram showing HepG2 cells stained with ab80717 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab80717, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • Formalin-fixed, paraffin-embedded human prostate stained with ab80717 at a 1/1500 dilution using alkaline phosphatase-conjugate and fast red chromogen. Note intense cytoplasmic staining of glandular epithelial cells.

Anti-PSAP antibody [PASE/4LJ] (ab80717)参考文献

This product has been referenced in:
  • Haines AM  et al. A novel hybridoma antibody (PASE/4LJ) to human prostatic acid phosphatase suitable for immunohistochemistry. Br J Cancer 60:887-92 (1989). ICC/IF, IHC-P ; Human . Read more (PubMed: 2605098) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab80717.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"