概述

  • 产品名称Anti-Prune homolog 2抗体
  • 描述
    兔多克隆抗体to Prune homolog 2
  • 特异性Reacts specifically with human 299 kDa Prune homolog 2 protein
  • 经测试应用适用于: IHC-Fr, ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide derived from Cter domain of human Prune homolog 2.

  • 阳性对照
    • This antibody gave a positive result when used in the following formaldehyde fixed cell lines: PC3

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液Preservative: None
    Constituents: Whole serum
  • 纯度Whole antiserum
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab80262 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr 1/100 - 1/500.
ICC/IF 1/200.
WB 1/500 - 1/5000. Detects a band of approximately 299 kDa.

靶标

  • 相关性There are 2 named isoforms produced by alternative splicing.
  • 细胞定位Cytoplasmic
  • 数据库链接
  • 别名
    • A214N16 3 antibody
    • BCH motif containing molecule at the carboxyl terminal region 1 antibody
    • BMCC1 antibody
    • BNIP2 motif containing molecule at the carboxyl terminal region 1 antibody
    • BNIPXL antibody
    • C9orf65 antibody
    • chromosome 9 open reading frame 65 antibody
    • prune homolog 2 (Drosophila) antibody
    • Prune2 antibody
    see all

Anti-Prune homolog 2 antibody 图像

  • ICC/IF image of ab80262 stained PC3 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab80262 at 1/200 dilution overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

Anti-Prune homolog 2 antibody (ab80262)参考文献

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