Anti-PRUNE抗体(ab88613)

概述

  • 产品名称Anti-PRUNE抗体
    参阅全部 PRUNE 一抗
  • 描述
    小鼠多克隆抗体to PRUNE
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Full length human PRUNE protein (NP_067045.1).

  • 阳性对照
    • WB: Raw 264.7 cell lysate, NIH/3T3 cell lysate, and PRUNE transfected 293T cell lysate ICC/IF: HeLa cell

性能

应用

Our Abpromise guarantee covers the use of ab88613 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 50 kDa.
ICC/IF Use a concentration of 10 µg/ml.

靶标

  • 功能Phosphodiesterase (PDE) that has higher activity toward cAMP than cGMP, as substrate. Plays a role in cell proliferation, is able to induce cell motility and acts as a negative regulator of NME1.
  • 组织特异性Ubiquitously expressed. Seems to be overexpressed in aggressive sarcoma subtypes, such as leiomyosarcomas and malignant fibrous histiocytomas (MFH) as well as in the less malignant liposarcomas.
  • 序列相似性Belongs to the PPase class C family. Prune subfamily.
  • 细胞定位Cytoplasm. Nucleus. Cell junction > focal adhesion. In some transfected cells a nuclear staining is also observed.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DRES-17 antibody
    • DRES17 antibody
    • Drosophila-related expressed sequence 17 antibody
    • hPrune antibody
    • HTcD37 antibody
    • Protein prune homolog antibody
    • PRUNE antibody
    • Prune homolog antibody
    • PRUNE like protein antibody
    • PRUNE_HUMAN antibody
    • TcD37 homolog antibody
    see all

Anti-PRUNE antibody 图像

  • Anti-PRUNE antibody (ab88613) at 1 µg/ml + Raw 264.7 cell lysate at 50 µg

    Predicted band size : 50 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
  • Anti-PRUNE antibody (ab88613) at 1 µg/ml + NIH/3T3 cell lysate at 50 µg

    Predicted band size : 50 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-PRUNE antibody (ab88613) at 1 µg/ml

    Lane 1 : PRUNE transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 50 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
  • ab88613, at 10 µg/ml, staining PRUNE in HeLa cell by Immunofluorescence.

Anti-PRUNE antibody (ab88613)参考文献

ab88613 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Dog Cell (canine progenitor epidermal keratinocytes (CPEK))
Permeabilization Yes - 0.3% Triton X-100
Specification canine progenitor epidermal keratinocytes (CPEK)
Blocking step BioGenex Power Block 10 mins, then 10% Normal Donkey Serum 20 minutes as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Paraformaldehyde
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提交于 Jan 11 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Dog Tissue sections (Skin)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate 10mM, 0.05% Tween 20, pH 6.0
Permeabilization Yes - 0.05% Tween 20
Specification Skin
Blocking step BioGenex Power Block 10 minutes, then 10% Normal Donkey Serum 20 minutes as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative Formaldehyde
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Abcam user community

Verified customer

提交于 Nov 24 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"