Synthetic peptide corresponding to Human PRSS3 aa 20-48 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
Database link: P35030-1
MDA-MB231 cell line lysates; Human pancreas tissue
Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
Preservative: 0.09% Sodium Azide
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Immunogen affinity purified
This antibody is purified through a protein A column, followed by peptide affinity purification.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Predicted molecular weight: 33 kDa.
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Digestive protease specialized for the degradation of trypsin inhibitors. In the ileum, may be involved in defensin processing, including DEFA5.
Expressed in pancreas and brain. Also expressed in Paneth cells, at the base of small intestinal crypts.
Belongs to the peptidase S1 family.
Contains 1 peptidase S1 domain.
Information by UniProt
Brain trypsinogen antibody
Western blot - Anti-PRSS3 antibody (ab107430)
Anti-PRSS3 antibody (ab107430) at 1/100 dilution + MDA-MB231 cell line lysates at 35 µg
Predicted band size: 33 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRSS3 antibody (ab107430)
ab107430, at a 1/50 dilution, staining PRSS3 in formalin fixed and paraffin embedded Human pancreas tissue by Immunohistochemistry. Detection utilised peroxidase conjugation of the secondary antibody and DAB staining.
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