antigen sequence: QCQAKAEEVC LPTCQHPCQD KCLVQAQEVC LSQCQESSQE KCPQQGQEPY LPPCQDQCPP QCAEPCQELF QTKCVEVCPQ KVQEKCSSPG, corresponding to amino acids 24-113 of Human NKIAMRE (UniProt ID: Q5T870).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-PRR9 antibody (ab121953)
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121953 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKIAMRE antibody (ab121953)
ab121953, at a 1/300 dilution staining NKIAMRE in paraffin emebedded Human placenta tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
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