Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
Synthetic peptide corresponding to a region within internal sequence amino acids 288-337 (PGKSQMDLRK LLRKVDVERS PGGTPLTNKE NMETGTGLTP VMTQALRRKF) of Human PRR11 (NP_060774).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
Concentration information loading...
Immunogen affinity purified
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The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Predicted molecular weight: 40 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Ubiquitinated (Probable). Rapidly degraded by the proteasome; degradation may involve FBXW7-specific phosphorylated phosphodegron motifs.
Information by UniProt
- FLJ11029 antibody
- Homo sapiens proline rich 11 antibody
- Proline rich protein 11 antibody
Western blot - PRR11 antibody (ab84565)
Anti-PRR11 antibody (ab84565) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µgSecondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilutionPredicted band size:
40 kDaObserved band size:
39 kDa (why is the actual band size different from the predicted?
)Additional bands at:
24 kDa. We are unsure as to the identity of these extra bands.
Gel concentration: 12%
has not yet been referenced specifically in any publications.
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