ELISA, WB, IHC-P more details
Synthetic peptide (Human) conjugated to KLH, selected from the sequence of Human PRPK within amino acids 200-253.
Mouse thymus tissue lysate, Human breast carcinoma.
存放说明 Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
存储溶液 Preservative: 0.09% Sodium Azide
Concentration information loading...
纯度 Protein G purified
纯化说明 This antibody was purified through a protein G column and eluted out with both high and low pH buffers. It was neutralized immediately after elution, followed by dialysis against PBS.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
1/50 - 1/100.
功能 Protein kinase that phosphorylates 'Ser-15' of p53/TP53 protein and may therefore participate in its activation.
组织特异性 Highly expressed in testis. Weakly expressed in heart kidney and spleen.
序列相似性 Belongs to the protein kinase superfamily. Contains 1 protein kinase domain.
Information by UniProt
Nori 2 antibody
Nori 2p antibody
Anti-PRPK antibody 图像
Western blot - PRPK antibody (ab37606)
Anti-PRPK antibody (ab37606) at 1/100 dilution + Mouse thymus tissue lysate Predicted band size : 28 kDa Observed band size : 28 kDa Additional bands at : 15-20 kDa,65 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PRPK antibody (ab37606)
ab37606, at 1/50 dilution, staining PRPK in Human breast carcinoma by Immunohistochemistry, Formalin fixed Paraffin embedded tissue. ab37606 was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
Anti-PRPK antibody (ab37606)参考文献
This product has been referenced in:
et al. Rab35 Functions in Axon Elongation Are Regulated by P53-Related Protein Kinase in a Mechanism That Involves Rab35 Protein Degradation and the Microtubule-Associated Protein 1B. J Neurosci 36:7298-313 (2016).
Read more (PubMed: 27383602) »
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"