概述

  • 产品名称Anti-PRPF8抗体
    参阅全部 PRPF8 一抗
  • 描述
    兔多克隆抗体to PRPF8
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Cow
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 2300 to the C-terminus of Human PRPF8.

    (Peptide available as ab87434.)

  • 阳性对照
    • This antibody gave a positive signal in the following lysates: HeLa Whole Cell lysate; HeLa Nuclear Lysate; NIH 3T3 Whole Cell lysate; A431 Whole Cell Lysate; MEF1 Whole Cell Lysate.

性能

应用

Our Abpromise guarantee covers the use of ab87433 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 250 kDa (predicted molecular weight: 274 kDa).
ICC/IF Use a concentration of 1 µg/ml.

靶标

  • 功能Central component of the spliceosome, which may play a role in aligning the pre-mRNA 5'- and 3'-exons for ligation. Interacts with U5 snRNA, and with pre-mRNA 5'-splice sites in B spliceosomes and 3'-splice sites in C spliceosomes.
  • 组织特异性Widely expressed.
  • 疾病相关Defects in PRPF8 are the cause of retinitis pigmentosa type 13 (RP13) [MIM:600059]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP13 inheritance is autosomal dominant.
  • 序列相似性Contains 1 MPN (JAB/Mov34) domain.
  • 结构域The MPN domain has structural similarity with viral ribonucleases and RNase H, but unlike RNases, it does not bind any metal ions.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Nucleus speckle.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 220 kDa U5 snRNP specific protein antibody
    • 220 kDa U5 snRNP-specific protein antibody
    • Apoptosis regulated protein 1 antibody
    • Apoptosis regulated protein 2 antibody
    • HPRP8 antibody
    • p220 antibody
    • Pre mRNA processing factor 8 antibody
    • Pre mRNA-processing factor 8, S. cerevisiae, homolog of antibody
    • Pre-mRNA-processing-splicing factor 8 antibody
    • Precursor mRNA processing protein antibody
    • PRP8 antibody
    • PRP8 homolog antibody
    • PRP8 pre mRNA processing factor 8 homolog antibody
    • PRP8_HUMAN antibody
    • PRPC8 antibody
    • Prpf8 antibody
    • Retinitis pigmentosa 13 (autosomal dominant) antibody
    • RP13 antibody
    • SNRNP220 antibody
    • Splicing factor Prp8 antibody
    • U5 snRNP specific protein antibody
    • U5 snRNP specific protein (220 kD), ortholog of S. cerevisiae Prp8p antibody
    see all

Anti-PRPF8 antibody 图像

  • All lanes : Anti-PRPF8 antibody (ab87433) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
    Lane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 4 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 5 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 274 kDa
    Observed band size : 250 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 100 kDa,175 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab87433 stained MCF-7 cells. The cells were 100% Methanol fixed (5 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab87433, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% Methanol fixed (5 min) Hek293, and HepG2 cells at 1µg/ml.

Anti-PRPF8 antibody (ab87433)参考文献

This product has been referenced in:
  • Ammon T  et al. The conserved ubiquitin-like protein Hub1 plays a critical role in splicing in human cells. J Mol Cell Biol 6:312-23 (2014). Human . Read more (PubMed: 24872507) »

See 1 Publication for this product

Product Wall

Thank you for your reply and your patience while I was confirming that I had the correct order information.

As requested I am sending ab72456 as a free of charge replacement for ab87433 and below is the testing discount code for trying this a...

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I think I have found your order from Cedarlane, but I just wanted to double check with your first. The order I have found, has us shipping the product on 26th March 2012 and so you would have received the product a f...

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Thank you for your reply.

Did you purchase the antibody directly from Abcam or did you purchase it through one of our distributors? The number you provided is not an actual Abcam order number and so I wanted to double check with you. If you d...

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Thank you for your reply.

I would be happy to send you ab72456 as a free of charge replacement for ab87433 and the discount code. To be able to send the free of charge replacement, it would be very helpful if you could send me the Abcam order...

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Thank you for contacting Abcam.

I am sorry about the issues you have been having with ab87433 in IF. Under our Abpromise, we do guarantee that the antibody should work on human tissue in IF. Since it is not and I do not think that any protoco...

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