概述

  • 产品名称Anti-PRPF8抗体[2834C1a]
    参阅全部 PRPF8 一抗
  • 描述
    小鼠单克隆抗体[2834C1a] to PRPF8
  • 经测试应用适用于: Flow Cyt, Dot blot, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Recombinant fragment (Human)

  • 阳性对照
    • NIH3T3 whole cell lysate, HeLa whole cell lysate This antibody gave a positive result in IHC in the following FFPE tissue: Human normal cervix.

性能

应用

Our Abpromise guarantee covers the use of ab51366 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Dot blot Use at an assay dependent concentration.
WB 1/100. Predicted molecular weight: 273 kDa.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Central component of the spliceosome, which may play a role in aligning the pre-mRNA 5'- and 3'-exons for ligation. Interacts with U5 snRNA, and with pre-mRNA 5'-splice sites in B spliceosomes and 3'-splice sites in C spliceosomes.
  • 组织特异性Widely expressed.
  • 疾病相关Defects in PRPF8 are the cause of retinitis pigmentosa type 13 (RP13) [MIM:600059]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP13 inheritance is autosomal dominant.
  • 序列相似性Contains 1 MPN (JAB/Mov34) domain.
  • 结构域The MPN domain has structural similarity with viral ribonucleases and RNase H, but unlike RNases, it does not bind any metal ions.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Nucleus speckle.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 220 kDa U5 snRNP specific protein antibody
    • 220 kDa U5 snRNP-specific protein antibody
    • Apoptosis regulated protein 1 antibody
    • Apoptosis regulated protein 2 antibody
    • HPRP8 antibody
    • p220 antibody
    • Pre mRNA processing factor 8 antibody
    • Pre mRNA-processing factor 8, S. cerevisiae, homolog of antibody
    • Pre-mRNA-processing-splicing factor 8 antibody
    • Precursor mRNA processing protein antibody
    • PRP8 antibody
    • PRP8 homolog antibody
    • PRP8 pre mRNA processing factor 8 homolog antibody
    • PRP8_HUMAN antibody
    • PRPC8 antibody
    • Prpf8 antibody
    • Retinitis pigmentosa 13 (autosomal dominant) antibody
    • RP13 antibody
    • SNRNP220 antibody
    • Splicing factor Prp8 antibody
    • U5 snRNP specific protein antibody
    • U5 snRNP specific protein (220 kD), ortholog of S. cerevisiae Prp8p antibody
    see all

Anti-PRPF8 antibody [2834C1a] 图像

  • IHC image of PRPF8 staining in Human Normal Cervix formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab51366, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-PRPF8 antibody [2834C1a] (ab51366) at 1/100 dilution + HeLa whole cell lysate at 25 µg

    Secondary
    Anti mouse IgG antibody at 1/2500 dilution

    Predicted band size : 273 kDa
    Observed band size : 250 kDa (why is the actual band size different from the predicted?)
  • Anti-PRPF8 antibody [2834C1a] (ab51366) at 1/100 dilution + NIH3T3 whole cell lysate at 25 µg

    Secondary
    Anti mouse IgG antibody at 1/2500 dilution

    Predicted band size : 273 kDa
    Observed band size : 250 kDa (why is the actual band size different from the predicted?)
  • Overlay histogram showing HeLa cells stained with ab51366 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab51366, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-PRPF8 antibody [2834C1a] (ab51366)参考文献

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