概述

  • 产品名称Anti-PRPF31抗体
    参阅全部 PRPF31 一抗
  • 描述
    山羊多克隆抗体to PRPF31
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Dog
  • 免疫原

    Synthetic peptide:

    KELGNSLDKCKNNEN

    , corresponding to internal sequence amino acids 140-154 of Human PRPF31

  • 阳性对照
    • HeLa cell lysate.

性能

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应用

Our Abpromise guarantee covers the use of ab58588 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
  • 应用说明Peptide ELISA: antibody detection limit dilution 1:128,000.

    WB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 55 kDa).

    An additional band of unknown identity was also consistently observed at 35kDa. This band was successfully blocked by incubation with the immunising peptide. Also a band of 28kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Involved in pre-mRNA splicing. Required for U4/U6.U5 tri-snRNP formation.
    • 组织特异性Ubiquitously expressed.
    • 疾病相关Defects in PRPF31 are the cause of retinitis pigmentosa type 11 (RP11) [MIM:600138]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP11 inheritance is autosomal dominant.
    • 序列相似性Belongs to the PRP31 family.
      Contains 1 Nop domain.
    • 结构域Interacts with the snRNP via the Nop domain.
      The coiled coil domain is formed by two non-contiguous helices.
    • 细胞定位Nucleus speckle. Nucleus > Cajal body. Predominantly found in speckles and in Cajal bodies.
    • Information by UniProt
    • 数据库链接
    • 别名
      • DKFZp566J153 antibody
      • hPrp 31 antibody
      • hPrp31 antibody
      • NY BR 99 antibody
      • Pre mRNA processing factor 31 antibody
      • Pre mRNA processing factor 31 homolog (yeast) antibody
      • Pre mRNA processing factor 31 homolog antibody
      • Pre-mRNA-processing factor 31 antibody
      • Precursor mRNA-processing factor 31, S. cerevisiae, homolog of antibody
      • Protein 61K antibody
      • PRP 31 antibody
      • PRP31 antibody
      • PRP31 pre mRNA processing factor 31 homolog (yeast) antibody
      • PRP31 pre mRNA processing factor 31 homolog antibody
      • PRP31_HUMAN antibody
      • PRPF 31 antibody
      • prpf31 antibody
      • RP 11 antibody
      • RP11 antibody
      • Serologically defined breast cancer antigen NY BR 99 antibody
      • Serologically defined breast cancer antigen NY-BR-99 antibody
      • SNRNP61 antibody
      • U4/U6 small nuclear ribonucleoprotein Prp31 antibody
      • U4/U6 snRNP 61 kDa protein antibody
      see all

    Anti-PRPF31 antibody 图像

    • All lanes : Anti-PRPF31 antibody (ab58588) at 1 µg/ml

      Lane 1 : HeLa cell lysate (35µg protein in RIPA buffer) without blocking with the immunizing peptide
      Lane 2 : HeLa cell lysate (35µg protein in RIPA buffer) with blocking with the immunizing peptide


      Predicted band size : 55 kDa
      Observed band size : 60 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 28 kDa,35 kDa. We are unsure as to the identity of these extra bands.

    Anti-PRPF31 antibody (ab58588)参考文献

    ab58588 has not yet been referenced specifically in any publications.

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