Anti-PRPF3抗体(ab50386)

概述

  • 产品名称Anti-PRPF3抗体
    参阅全部 PRPF3 一抗
  • 描述
    兔多克隆抗体to PRPF3
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Dog, Pig
  • 免疫原

    A region within synthetic peptide: VDKLFEAVEE GRSSRHSKSS SDRSRKRELK EVFGDDSEIS KESSGVKKRR, corresponding to amino acids 63-112 of Human PRPF3

  • 阳性对照
    • HepG2 cell lysate

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab50386 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/62500.

Titre using peptide based assay 1/62500.

靶标

Anti-PRPF3 antibody 图像

  • Lane 1 : Molecular weight markers
    Lane 2 : Anti-PRPF3 antibody (ab50386) at 0.5 µg/ml

    Lane 1 : As above
    Lane 2 : HepG2 cell lysate at 10 µg

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 77 kDa
    Observed band size : 77 kDa

Anti-PRPF3 antibody (ab50386)参考文献

ab50386 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - other (Hek 293T)
Gel Running Conditions Reduced Denaturing (10%)
Loading amount 1e+007 cells
Specification Hek 293T
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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提交于 Nov 01 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (U2OS ATCC)
Permeabilization Yes - 0.5% Triton X-100 in PBS 1X
Specification U2OS ATCC
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 23°C
Fixative Paraformaldehyde
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提交于 Oct 07 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"