概述

  • 产品名称
  • 描述
    兔多克隆抗体to PRP6
  • 经测试应用
    适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Chicken, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis, Platypus
  • 免疫原

    Synthetic peptide, corresponding to a region between residue 1 and 50 of Human PRP6 (NP_036601.2)

  • 阳性对照
    • HeLa, 293T and NIH3T3 whole cell lysate

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG

应用

Our Abpromise guarantee covers the use of ab99292 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 107 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能
    Involved in pre-mRNA splicing. May act in the tri-snRNP complex as a bridging factor between U5 and U4/U6 snRNPs in the late step of spliceosome assembly. May be necessary for tri-snRNP formation.
  • 序列相似性
    Contains 9 HAT repeats.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Androgen receptor N terminal domain transactivating protein 1 antibody
    • ANT 1 antibody
    • ANT1 antibody
    • C20orf14 antibody
    • hPrp6 antibody
    • p102 U5 small nuclear ribonucleoprotein particle binding protein antibody
    • pre-mRNA processing factor 6 antibody
    • Pre-mRNA-processing factor 6 antibody
    • PRP6 homolog antibody
    • PRP6 pre mRNA processing factor 6 homolog antibody
    • PRP6_HUMAN antibody
    • PRPF6 antibody
    • Putative mitochondrial outer membrane protein import receptor antibody
    • TOM antibody
    • U5 snRNP-associated 102 kDa protein antibody
    • U5-102 kDa protein antibody
    • U5-102K antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling PRP6 with ab99292 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-PRP6 antibody (ab99292) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg


    Predicted band size : 107 kDa


    Exposure time : 30 seconds
  • Lane 1 : Detection of Human PRP6 by immunoprecipitation from HeLa (1 mg for IP, 20% of IP loaded), using 3µg/ml ab99292. Lane 2: Control IgG

文献

This product has been referenced in:
  • Cloutier P  et al. R2TP/Prefoldin-like component RUVBL1/RUVBL2 directly interacts with ZNHIT2 to regulate assembly of U5 small nuclear ribonucleoprotein. Nat Commun 8:15615 (2017). WB . Read more (PubMed: 28561026) »
  • Liu YC  et al. Cytoplasmic Viral RNA-Dependent RNA Polymerase Disrupts the Intracellular Splicing Machinery by Entering the Nucleus and Interfering with Prp8. PLoS Pathog 10:e1004199 (2014). WB ; Human . Read more (PubMed: 24968230) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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