概述

  • 产品名称Anti-PROX1抗体
    参阅全部 PROX1 一抗
  • 描述
    兔多克隆抗体to PROX1
  • 特异性We have had mixed results for use of this antibody in IHC-P. The abReview below from an external researcher reports good staining with this antibody with human heart tissue, but we have had mixed results with this antibody in internal QC testing in IHC-P. Thus, we are removing IHC-P as a guaranteed application and welcome any feedback from customers who have used this antibody in IHC-P.
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human PROX1.

    (Peptide available as ab38691.)

  • 阳性对照
    • This antibody gave a positive signal in the following human whole cell lysates : HepG2 and SHSY-5Y. HEK293 human whole cell lysate is a negative control lysate.

性能

应用

Our Abpromise guarantee covers the use of ab38692 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 2 µg/ml. Detects a band of approximately 105 kDa (predicted molecular weight: 83 kDa).

靶标

  • 功能Transcription factor involved in developmental processes such as cell fate determination, gene transcriptional regulation and progenitor cell regulation in a number of organs. Plays a critical role in embryonic development and functions as a key regulatory protein in neurogenesis and the development of the heart, eye lens, liver, pancreas and the lymphatic system. Involved in the regulation of the circadian rhythm. Represses: transcription of the retinoid-related orphan receptor RORG, transcriptional activator activity of RORA and RORG and the expression of RORA/G-target genes including core clock components: ARNTL/BMAL1, NPAS2 and CRY1 and metabolic genes: AVPR1A and ELOVL3.
  • 组织特异性Most actively expressed in the developing lens. Detected also in embryonic brain, lung, liver and kidney. In adult, it is more abundant in heart and liver than in brain, skeletal muscle, kidney and pancreas.
  • 序列相似性Belongs to the Prospero homeobox family.
    Contains 1 Prospero-type homeobox DNA-binding domain.
  • 结构域The prospero-type homeobox DNA-binding domain is essential for repression of RORG transcriptional activator activity.
  • 细胞定位Nucleus. RORG promotes its nuclear localization.
  • Information by UniProt
  • 数据库链接
  • 形式Prox1 is a specific and required regulator of the development of the lymphatic system and that the vascular and lymphatic systems develop independently. Sox18 directly activates Prox1 transcription by binding to its proximal promoter.
  • 别名
    • Homeobox prospero like protein antibody
    • Homeobox prospero like protein PROX1 antibody
    • Homeobox prospero-like protein PROX1 antibody
    • Prospero homeobox 1 antibody
    • Prospero homeobox protein 1 antibody
    • Prospero related homeobox 1 antibody
    • prospero-related homeobox gene 1 antibody
    • PROX 1 antibody
    • PROX-1 antibody
    • PROX1 antibody
    • PROX1_HUMAN antibody
    • zgc:111888 antibody
    see all

Anti-PROX1 antibody 图像

  • All lanes : Anti-PROX1 antibody (ab38692) at 1 µg/ml (3% Milk Block)

    Lane 1 : HepG2 (Human) Whole Cell Lysate (ab52257)
    Lane 2 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate (ab45968)
    Lane 3 : HEK293 (Human) Whole Cell Lysate (ab52256)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 83 kDa
    Observed band size : 95 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 25 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes

Anti-PROX1 antibody (ab38692)参考文献

This product has been referenced in:
  • Osborn AJ  et al. Activating PIK3CA alleles and lymphangiogenic phenotype of lymphatic endothelial cells isolated from lymphatic malformations. Hum Mol Genet 24:926-38 (2015). Read more (PubMed: 25292196) »
  • Davis JM  et al. Lymphatic endothelial differentiation in pulmonary lymphangioleiomyomatosis cells. J Histochem Cytochem 61:580-90 (2013). Human . Read more (PubMed: 23609227) »

See all 4 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (testis)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0
Permeabilization No
Specification testis
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Aug 18 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Dog Tissue sections (gut)
Antigen retrieval step Heat mediated
Specification gut
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Apr 29 2015



Es freut mich zu hören, dass Sie unser Angebot unseren Antikörper ab38692 in Schweinegewebezu testen, annehmen möchten. Wie am Telefon besprochen, können Sie den Gutscheincode zum Kauf eines weiteren Antikörpers einse...

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Vielen Dank für Ihren Anruf und für Ihr Interesse an unseren Produkten.

Leider sind Schwein und Maus oder Humanes Proteinfür Anti-Podoplanin / gp36 und CD31 nicht sehr homolog. Deshalb kann ich Ihnen auch keine Antikörper ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (mouse heart)
Loading amount 50 µg
Specification mouse heart
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Username

Abcam user community

Verified customer

提交于 Jul 27 2012

Thank you for contacting us.

The anti-PROX1 antibody ab38692 is $347 for 100 µg+ $41 shipping and handling in the US. The antibody comes as a liquid, 100 µg at 1mg/ml, so you receive 100 µl.

http://www.abcam.com/p...

Read More

Merci pour votre émail. Nous avons plusieurs alternatives pour cet anticorps, mais ça dépends dans quel application et sur quel espèce le client veux détecter le PROX I. Le ab33219 est probablement le meilleur choix de remplacement, pourvu que le clien...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (E9.5 EMBRYONIC PROEPICARDIUM AND ADJACENT LIVER BU)
Specification E9.5 EMBRYONIC PROEPICARDIUM AND ADJACENT LIVER BU
Fixative Paraformaldehyde
Permeabilization Yes - 1% Triton X-100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: RT°C
Username

Dr. Stephanie Cossette

Verified customer

提交于 Oct 05 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (P3 Dentate Gyrus)
Loading amount 50 µg
Specification P3 Dentate Gyrus
Gel Running Conditions Reduced Denaturing (8%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Ms. Xia Chen

Verified customer

提交于 Apr 01 2011

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Heart)
Specification Heart
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako Target Retrieval Solution
Permeabilization No
Blocking step 5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

提交于 Apr 08 2010

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"