概述

  • 产品名称Anti-Protocadherin 21抗体
    参阅全部 Protocadherin 21 一抗
  • 描述
    鸡多克隆抗体to Protocadherin 21
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow
  • 免疫原

    Synthetic peptide: V GSHVYTLN GT DPEGDPI SYH ISFDPS TRSV FSVDP TFGNI TLVE ELDRER, corresponding to N terminal amino acids 50-100 of Human Protocadherin 21

  • 阳性对照
    • E. coli-derived PCDH21 fusion protein

性能

应用

Our Abpromise guarantee covers the use of ab36669 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IHC-P
  • 应用说明IHC-P: Use at an assay dependent dilution (PMID 18477406).
    WB: 1/2000. Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Potential calcium-dependent cell-adhesion protein. May be required for the structural integrity of the outer segment (OS) of photoreceptor cells.
    • 序列相似性Contains 6 cadherin domains.
    • 翻译后修饰Undergoes proteolytic cleavage; produces a soluble 95 kDa N-terminal fragment and a 25 kDa cell-associated C-terminal fragment.
    • 细胞定位Cell membrane. Localized at the junction between the inner and outer segments of rod and cone photoreceptors cells. Confined to the base of the OS. Localized on the edges of nascent evaginating disks on the side of the OS opposite the connecting cilium. Expressed at postnatal day 2 at the apical tip of the rod photoreceptor cells, the site of the developing OS. Colocalized with rhodopsin between postnatal days 2 and 9 at the base of the growing OS region.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Cadherin-related family member 1 antibody
      • CDHR1 antibody
      • CDHR1_HUMAN antibody
      • CORD15 antibody
      • DKFZp434A132 antibody
      • KIAA1775 antibody
      • MT protocadherin antibody
      • PCDH 21 antibody
      • PCDH21 antibody
      • PCDH21 protein antibody
      • Photoreceptor cadherin antibody
      • prCAD antibody
      • Protocadherin-21 antibody
      • RP65 antibody
      see all

    Anti-Protocadherin 21 antibody 图像

    • Anti-Protocadherin 21 antibody (ab36669) at 1/2000 dilution + E.coli derived Human PCDH21 fusion protein at 2.5 µg

      Secondary
      Goat anti-IgY at 1/1000 dilution

      Predicted band size : 82 kDa

    Anti-Protocadherin 21 antibody (ab36669)参考文献

    This product has been referenced in:
    • Hardman MJ & Ashcroft GS Estrogen, not intrinsic aging, is the major regulator of delayed human wound healing in the elderly. Genome Biol 9:R80 (2008). IHC-P ; Human . Read more (PubMed: 18477406) »

    See 1 Publication for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (mouse olfactory bulb)
    Specification mouse olfactory bulb
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer
    Permeabilization Yes - 0.3% TX100
    Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
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    提交于 Apr 09 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"