重组
RabMAb

Anti-Prothrombin抗体[EPR5188] (ab109087)

概述

  • 产品名称
    Anti-Prothrombin抗体[EPR5188]
    参阅全部 Prothrombin 一抗
  • 描述
    兔单克隆抗体[EPR5188] to Prothrombin
  • 经测试应用
    适用于: WB, IPmore details
    不适用于: Flow Cyt,ICC or IHC-P
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human Prothrombin.

  • 阳性对照
    • Human serum, plasma, and platelet lysates.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 纯度
    Tissue culture supernatant
  • 克隆
    单克隆
  • 克隆编号
    EPR5188
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab109087 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 70 kDa.
IP 1/10 - 1/100.
  • 应用说明
    Is unsuitable for Flow Cyt,ICC or IHC-P.
  • 靶标

    • 功能
      Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing.
    • 组织特异性
      Expressed by the liver and secreted in plasma.
    • 疾病相关
      Defects in F2 are the cause of factor II deficiency (FA2D) [MIM:613679]. It is a very rare blood coagulation disorder characterized by mucocutaneous bleeding symptoms. The severity of the bleeding manifestations correlates with blood factor II levels.
      Genetic variations in F2 may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.
      Defects in F2 are a cause of susceptibility to thrombosis (THR) [MIM:188050]. It is a multifactorial disorder of hemostasis characterized by abnormal platelet aggregation in response to various agents and recurrent thrombi formation. Note=A common genetic variation in the 3-prime untranslated region of the prothrombin gene is associated with elevated plasma prothrombin levels and an increased risk of venous thrombosis.
    • 序列相似性
      Belongs to the peptidase S1 family.
      Contains 1 Gla (gamma-carboxy-glutamate) domain.
      Contains 2 kringle domains.
      Contains 1 peptidase S1 domain.
    • 翻译后修饰
      The gamma-carboxyglutamyl residues, which bind calcium ions, result from the carboxylation of glutamyl residues by a microsomal enzyme, the vitamin K-dependent carboxylase. The modified residues are necessary for the calcium-dependent interaction with a negatively charged phospholipid surface, which is essential for the conversion of prothrombin to thrombin.
    • 细胞定位
      Secreted > extracellular space.
    • Information by UniProt
    • 数据库链接
    • 形式
      Cleaved into the following 4 chains: 1. Activation peptide fragment 1 2. Activation peptide fragment 2 3. Thrombin light chain 4. Thrombin heavy chain
    • 别名
      • coagulation factor II (thrombin) antibody
      • Coagulation factor II antibody
      • F2 antibody
      • Factor II antibody
      • Prepro coagulation factor II antibody
      • Prothrombin antibody
      • prothrombin B-chain antibody
      • PT antibody
      • RPRGL2 antibody
      • serine protease antibody
      • THPH1 antibody
      • THRB antibody
      • THRB_HUMAN antibody
      • Thrombin heavy chain antibody
      see all

    图片

    • All lanes : Anti-Prothrombin antibody [EPR5188] (ab109087) at 1/1000 dilution

      Lane 1 : Human serum lysate
      Lane 2 : Human plasma lysate
      Lane 3 : Human platelet lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 70 kDa

    文献

    This product has been referenced in:
    • Wu Z  et al. Identification of Potential Biomarkers for Rhegmatogenous Retinal Detachment Associated with Choroidal Detachment by Vitreous iTRAQ-Based Proteomic Profiling. Int J Mol Sci 17:N/A (2016). WB . Read more (PubMed: 27941623) »

    See 1 Publication for this product

    客户评价及客户问答

    Thank you for contacting us.

    We do have not tested antibodies which recognizes carboxylated form of Prothrombin.

    ab49709 seems a closest match.

    I hope this information is helpful to you. Please do not hesitate to contact...

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    Thank you for contacting us.

    I have checked all prothrombin antibodies in our catalog, but for none of them we know for sure that they will bind specifically to the carboxyglutamated form.

    As for ab109087, I found out that the immu...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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