概述

  • 产品名称
    Proteinase K
  • 经测试应用
    适用于: IHC-P, In situ hybridizationmore details
  • 常规说明

    STAINING PROTOCOL:

    1. IHC procedure: Incubate tissue section for 5 to 7 minutes at room temperature; Rinse and proceed with immunostaining.
    2. General ISH procedure: Incubate tissue section for 15 minutes at 37oC; Rinse and proceed with hybridization.

     

    ab64220 is provided as a single component, ready-to-use solution.

性能

应用

Our Abpromise guarantee covers the use of ab64220 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent dilution.
In situ hybridization Use at an assay dependent dilution.

文献

This product has been referenced in:
  • Plebanek MP  et al. Pre-metastatic cancer exosomes induce immune surveillance by patrolling monocytes at the metastatic niche. Nat Commun 8:1319 (2017). IHC-P ; Mouse . Read more (PubMed: 29105655) »
  • Li H  et al. The superior regenerative potential of muscle-derived stem cells for articular cartilage repair is attributed to high cell survival and chondrogenic potential. Mol Ther Methods Clin Dev 3:16065 (2016). IHC ; Mouse . Read more (PubMed: 27990446) »

See all 2 Publications for this product

客户评价及客户问答

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fast and easy

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Proteinase K digestion has worked well for cytokeratin 20 (ab854) staining.
The enzyme is ready to use and requires a maximum of 15 minutes for digestion.
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提交于 Feb 08 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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