Anti-Proteinase Activated Receptor 4抗体(ab56359)

概述

  • 产品名称
    Anti-Proteinase Activated Receptor 4抗体
    参阅全部 Proteinase Activated Receptor 4 一抗
  • 描述
    小鼠单克隆抗体to Proteinase Activated Receptor 4
  • 经测试应用
    适用于: ICC/IF, WB, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment: GGTQTPSVYD ESGSTGGGDD STPSILPAPR GYPGQVCAND SDTLELPDSS RALLLGWVPT RLVPA, corresponding to amino acids 18-83 of Human Proteinase Activated Receptor 4

  • 常规说明

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 克隆
    单克隆
  • 同种型
    IgG1
  • 轻链类型
    kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab56359 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 - 5 µg/ml.

This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能
    Receptor for activated thrombin or trypsin coupled to G proteins that stimulate phosphoinositide hydrolysis. May play a role in platelets activation.
  • 组织特异性
    Widely expressed, with highest levels in lung, pancreas, thyroid, testis and small intestine. Not expressed in brain, kidney, spinal cord and peripheral blood leukocytes. Also detected in platelets.
  • 序列相似性
    Belongs to the G-protein coupled receptor 1 family.
  • 翻译后修饰
    A proteolytic cleavage generates a new N-terminus that functions as a tethered ligand.
  • 细胞定位
    Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Coagulation factor II (thrombin) receptor like 3 antibody
    • Coagulation factor II receptor like 3 antibody
    • Coagulation factor II receptor-like 3 antibody
    • F2RL3 antibody
    • PAR 4 antibody
    • PAR-4 antibody
    • PAR4 antibody
    • PAR4_HUMAN antibody
    • protease activated receptor 4 antibody
    • protease activated receptor4 antibody
    • Proteinase activated receptor 4 antibody
    • Proteinase-activated receptor 4 antibody
    • Thrombin receptor like 3 antibody
    • Thrombin receptor-like 3 antibody
    see all

图片

  • Western blot against tagged recombinant protein immunogen using ab56359 Proteinase Activated Receptor 4 antibody at 1ug/ml. Predicted band size of immunogen is 33 kDa
  • ICC/IF image of ab56359 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56359, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing A549 cells stained with ab56359 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56359, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

文献

ab56359 has not yet been referenced specifically in any publications.

客户评价及客户问答

I am doing very well, and yourself?

I have looked at the suggested replacement antibody of ab66103, and I think as long as the end user is OK with it being a rabbit polyclonal, instead of a mouse monoclonal antibody, then they should have no i...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册