Anti-Protein phosphatase 1 inhibitor subunit 2抗体(ab73948)

概述

  • 产品名称Anti-Protein phosphatase 1 inhibitor subunit 2抗体
    参阅全部 Protein phosphatase 1 inhibitor subunit 2 一抗
  • 描述
    兔多克隆抗体to Protein phosphatase 1 inhibitor subunit 2
  • 特异性ab73948 detects endogenous levels of total Protein phosphatase 1 inhibitor subunit 2 protein.
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic non-phosphopeptide derived from human Protein phosphatase 1 inhibitor subunit 2 around the phosphorylation site of serine 120 and serine 121 (Q-E-SP-SP-G-E).

  • 阳性对照
    • Human breast carcinoma tissue. Extracts from Jurkat cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab73948 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 23 kDa).
ELISA 1/10000.
IHC-P 1/50 - 1/100.

靶标

Anti-Protein phosphatase 1 inhibitor subunit 2 antibody 图像

  • All lanes : Anti-Protein phosphatase 1 inhibitor subunit 2 antibody (ab73948) at 1/500 dilution

    Lane 1 : Extracts from Jurkat cells
    Lane 2 : Extracts from Jurkat cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 23 kDa
    Observed band size : 25 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using ab73948, at 1/50 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.

Anti-Protein phosphatase 1 inhibitor subunit 2 antibody (ab73948)参考文献

ab73948 has not yet been referenced specifically in any publications.

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