Anti-Protein L抗体(FITC) (ab63504)

概述

  • 产品名称Anti-Protein L抗体(FITC)
    参阅全部 Protein L 一抗
  • 描述
    鸡多克隆抗体to Protein L (FITC)
  • 偶联物FITC. Ex: 493nm, Em: 528nm
  • 经测试应用适用于: IHC-Frmore details
  • 种属反应性
    Reacts with bacteria.
  • 免疫原

    Purified recombinant protein L.

性能

应用

Our Abpromise guarantee covers the use of ab63504 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr 1/500 - 1/1000.

靶标

  • 相关性Protein L is a 36,000 dalton immunoglobulin-binding protein isolated from the bacteria Peptostreptococcus magnus. Unlike Protein A and Protein G, which bind to the Fc region of immunoglobulins (antibodies), Protein L binds antibodies through light chain interactions. Since no part of the heavy chain is involved in the binding interaction, Protein L binds a wider range of antibody classes than Protein A or G. Protein L binds to representatives of all antibody classes, including IgG, IgM, IgA, IgE and IgD. Single chain variable fragments (ScFv) and Fab fragments also bind to Protein L. Despite this wide binding range, Protein L is not a universal antibody-binding protein. Protein L binding is restricted to those antibodies that contain kappa light chains and it is only effective in binding certain subtypes of kappa light chains - about 65% of human immunoglobulins carry kappa light chains. Given these specific requirements for effective binding, the main application for immobilized Protein L is purification of monoclonal antibodies from ascites or cell culture supernatant that are known to have the kappa light chain. Protein L is extremely useful for purification of VLkappa-containing monoclonal antibodies from culture supernatant because it does not bind bovine immunoglobulins, which are often present in the media as a serum supplement. Also, Protein L does not interfere with the antigen-binding site of the antibody, making it useful for immunoprecipitation assays, even using IgM.
  • 细胞定位Cell surface
  • 别名
    • ProteinL antibody
    • RPL antibody

Anti-Protein L antibody (FITC) (ab63504)参考文献

ab63504 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry.

Currently, we have 4 antibodies against Protein L but none of them have been tested in immunoprecipitation:

ab63503, ab63504, ab63505, ab63506

If your customer would like to test any of these an...

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Thank you for contacting us. Unfortunately, I would not recommend using a chicken antibody against a different target (such as ab1394 or ab63504), as it cannot be guaranteed that they do not react with any human antigen. I would rather suggest to use a...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"