兔单克隆抗体[EP1496Y] to Protein Kinase D2 (phospho S876)
This antibody detects Protein Kinase D2 when phosphorylated at Ser876.
Synthetic phospho-peptide corresponding to residues surrounding Serine 876 of Human Protein Kinase D2.
This product is a recombinant rabbit monoclonal antibody.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000. Detects a band of approximately 105 kDa (predicted molecular weight: 97 kDa).
Is unsuitable for Flow Cyt, IHC or ICC/IF.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress.
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.
Contains 1 PH domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain.
Phosphorylation of Ser-876 correlates with the activation status of the kinase.
Ser-706 is probably phosphorylated by PKC.
Cytoplasm. Membrane. Translocation to the cell membrane is required for kinase activation.
Information by UniProt
- DKFZp586E0820 antibody
- HSPC187 antibody
- KPCD2_HUMAN antibody
Western blot - Protein Kinase D2 (phospho S876) antibody [EP1496Y] (ab51251)
All lanes :
Anti-Protein Kinase D2 (phospho S876) antibody [EP1496Y] (ab51251) at 1/1000 dilutionLane 1 :
HeLa cell lysate, untreatedLane 2 :
HeLa cell lysate, treated with TPA
Lysates/proteins at 10 µg per lane.Secondary
Goat anti-Rabbit HRP labeled at 1/2000 dilutionPredicted band size :
97 kDaObserved band size :
105 kDa (why is the actual band size different from the predicted?
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"