RabMAb

Anti-Protein Kinase D2 (phospho S876)抗体[EP1496Y] (ab51251)

概述

  • 产品名称Anti-Protein Kinase D2 (phospho S876)抗体[EP1496Y]
    参阅全部 Protein Kinase D2 一抗
  • 描述
    兔单克隆抗体[EP1496Y] to Protein Kinase D2 (phospho S876)
  • 特异性This antibody detects Protein Kinase D2 when phosphorylated at Ser876.
  • 经测试应用适用于: WB, IPmore details
    不适用于: Flow Cyt or ICC/IF
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic phospho-peptide corresponding to residues surrounding Serine 876 of Human Protein Kinase D2.

  • 阳性对照
    • HeLa cell lysate.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab51251 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
  • 应用说明IP: 1/40.
    WB: 1/1000. Detects a band of approximately 105 kDa (predicted molecular weight: 97 kDa).

    Is unsuitable for Flow Cyt, IHC or ICC/IF.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress.
    • 组织特异性Widely expressed.
    • 序列相似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.
      Contains 1 PH domain.
      Contains 2 phorbol-ester/DAG-type zinc fingers.
      Contains 1 protein kinase domain.
    • 翻译后修饰Phosphorylation of Ser-876 correlates with the activation status of the kinase.
      Ser-706 is probably phosphorylated by PKC.
    • 细胞定位Cytoplasm. Membrane. Translocation to the cell membrane is required for kinase activation.
    • Information by UniProt
    • 数据库链接
    • 别名
      • DKFZp586E0820 antibody
      • HSPC187 antibody
      • KPCD2_HUMAN antibody
      • nPKC D2 antibody
      • nPKC-D2 antibody
      • PKD2 antibody
      • PRKD 2 antibody
      • Prkd2 antibody
      • Protein kinase D2 antibody
      • Serine/threonine protein kinase D2 antibody
      • Serine/threonine-protein kinase D2 antibody
      see all

    Anti-Protein Kinase D2 (phospho S876) antibody [EP1496Y] 图像

    • All lanes : Anti-Protein Kinase D2 (phospho S876) antibody [EP1496Y] (ab51251) at 1/1000 dilution

      Lane 1 : HeLa cell lysate, untreated
      Lane 2 : HeLa cell lysate, treated with TPA

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP labeled at 1/2000 dilution

      Predicted band size : 97 kDa
      Observed band size : 105 kDa (why is the actual band size different from the predicted?)

    Anti-Protein Kinase D2 (phospho S876) antibody [EP1496Y] (ab51251)参考文献

    This product has been referenced in:
    • Bernhart E  et al. Protein kinase D2 regulates migration and invasion of U87MG glioblastoma cells in vitro. Exp Cell Res 319:2037-48 (2013). Read more (PubMed: 23562655) »

    See 1 Publication for this product

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