存放说明 Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
存储溶液 Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration information loading...
纯度 Immunogen affinity purified
纯化说明 The antibody against non-phosphopeptide was removed by chromatography
using non-phosphopeptide corresponding to the phosphorylation site.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa).
功能 Converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress.
组织特异性 Widely expressed.
序列相似性 Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily. Contains 1 PH domain. Contains 2 phorbol-ester/DAG-type zinc fingers. Contains 1 protein kinase domain.
翻译后修饰 Phosphorylation of Ser-876 correlates with the activation status of the kinase. Ser-706 is probably phosphorylated by PKC.
细胞定位 Cytoplasm. Membrane. Translocation to the cell membrane is required for kinase activation.
Information by UniProt
Anti-Protein Kinase D2 (phospho S876) antibody 图像
Western blot - Protein Kinase D2 (phospho S876) antibody (ab59417)
All lanes : Anti-Protein Kinase D2 (phospho S876) antibody (ab59417) at 1/500 dilution Lane 1 : extracts from NIH/3T3cells treated with PMA (250ng/ml, 15mins) Lane 2 : extracts from NIH/3T3cells treated with PMA (250ng/ml, 15mins) with blocking phosphopeptide Predicted band size : 97 kDa Observed band size : 97 kDa Additional bands at : 90 kDa. We are unsure as to the identity of these extra bands.
Anti-Protein Kinase D2 (phospho S876) antibody (ab59417)参考文献
This product has been referenced in:
et al. Protein Kinase D1 Mediates Stimulation of DNA Synthesis and Proliferation in Intestinal Epithelial IEC-18 Cells and in Mouse Intestinal Crypts. J Biol Chem 286:511-20 (2011).
Read more (PubMed: 21051537) »
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"