Anti-Protein Kinase D2 (phospho S876)抗体(ab59417)

概述

  • 产品名称Anti-Protein Kinase D2 (phospho S876)抗体
    参阅全部 Protein Kinase D2 一抗
  • 描述
    兔多克隆抗体to Protein Kinase D2 (phospho S876)
  • 特异性Detects endogenous levels of PKD2 only when phosphorylated at serine 876
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic phosphopeptide derived from human PKD2 around the phosphorylation site of serine 876 (R-I-SP-V-L).

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab59417 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa).
ELISA 1/60000.

靶标

  • 功能Converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress.
  • 组织特异性Widely expressed.
  • 序列相似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.
    Contains 1 PH domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • 翻译后修饰Phosphorylation of Ser-876 correlates with the activation status of the kinase.
    Ser-706 is probably phosphorylated by PKC.
  • 细胞定位Cytoplasm. Membrane. Translocation to the cell membrane is required for kinase activation.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZp586E0820 antibody
    • HSPC187 antibody
    • KPCD2_HUMAN antibody
    • nPKC D2 antibody
    • nPKC-D2 antibody
    • PKD2 antibody
    • PRKD 2 antibody
    • Prkd2 antibody
    • Protein kinase D2 antibody
    • Serine/threonine protein kinase D2 antibody
    • Serine/threonine-protein kinase D2 antibody
    see all

Anti-Protein Kinase D2 (phospho S876) antibody 图像

  • All lanes : Anti-Protein Kinase D2 (phospho S876) antibody (ab59417) at 1/500 dilution

    Lane 1 : extracts from NIH/3T3cells treated with PMA (250ng/ml, 15mins)
    Lane 2 : extracts from NIH/3T3cells treated with PMA (250ng/ml, 15mins) with blocking phosphopeptide


    Predicted band size : 97 kDa
    Observed band size : 97 kDa
    Additional bands at : 90 kDa. We are unsure as to the identity of these extra bands.

Anti-Protein Kinase D2 (phospho S876) antibody (ab59417)参考文献

This product has been referenced in:
  • Sinnett-Smith J  et al. Protein Kinase D1 Mediates Stimulation of DNA Synthesis and Proliferation in Intestinal Epithelial IEC-18 Cells and in Mouse Intestinal Crypts. J Biol Chem 286:511-20 (2011). WB ; Rat . Read more (PubMed: 21051537) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab59417.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"