Anti-Protein Kinase D2抗体(ab59372)


  • 产品名称Anti-Protein Kinase D2抗体
    参阅全部 Protein Kinase D2 一抗
  • 描述
    兔多克隆抗体to Protein Kinase D2
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic non-phosphopeptide derived from Human Protein Kinase D2 around the phosphorylation site of serine 876 (R-I-SP-V-L).

  • 阳性对照
    • NIH/3T3 cells.


  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域


Our Abpromise guarantee covers the use of ab59372 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 97, 100 kDa (predicted molecular weight: 97 kDa).
ELISA 1/5000.
IHC-P Use a concentration of 1 µg/ml.


  • 功能Converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress.
  • 组织特异性Widely expressed.
  • 序列相似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.
    Contains 1 PH domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • 翻译后修饰Phosphorylation of Ser-876 correlates with the activation status of the kinase.
    Ser-706 is probably phosphorylated by PKC.
  • 细胞定位Cytoplasm. Membrane. Translocation to the cell membrane is required for kinase activation.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZp586E0820 antibody
    • HSPC187 antibody
    • KPCD2_HUMAN antibody
    • nPKC D2 antibody
    • nPKC-D2 antibody
    • PKD2 antibody
    • PRKD 2 antibody
    • Prkd2 antibody
    • Protein kinase D2 antibody
    • Serine/threonine protein kinase D2 antibody
    • Serine/threonine-protein kinase D2 antibody
    see all

Anti-Protein Kinase D2 antibody 图像

  • All lanes : Anti-Protein Kinase D2 antibody (ab59372) at 1/500 dilution

    Lane 1 : Extracts from NIH/3T3 cells, treated with PMA (250ng/ml, 15mins)
    Lane 2 : Extracts from NIH/3T3 cells, treated with PMA (250ng/ml, 15mins) with immunizing phosphopeptide

    Predicted band size : 97 kDa
    Observed band size : 97,100 kDa (why is the actual band size different from the predicted?)
  • Ab59372 staining human normal tonsil. Staining is localised to cytoplasmic compartment.
    Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Anti-Protein Kinase D2 antibody (ab59372)参考文献

ab59372 has not yet been referenced specifically in any publications.

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