蛋白G Agarose (High Affinity) (ab193258)
Key features and details
- Sample type: Ascites Fluid, Cell culture media, Cell culture supernatant, Serum
概述
-
产品名称
蛋白G Agarose (High Affinity) -
样品类型
Cell culture supernatant, Serum, Cell culture media, Ascites Fluid -
产品概述
Very high binding capacity (>30 mg IgG/mL). Minimal leaching of ligand. Suitable for column or batch purification of IgG, immunoprecipitation & ChIP (ab193258).
Contents:
Supplied as 50% slurry in 20% Ethanol.
Features:
High binding capacity: Binding of IgG ≥ 30 mg human or rabbit IgG/mL Protein G Agarose.
Minimal leaching of the ligand
Flow Rate Tested*: 2.89 mL/min.
*Test condition: = Calculations based on the time required to pass 18 mL of water through 2 mL settled beads (column diameter 1.5 cm).
Maximum Flow Rate**: 1800 cm/hr; minimum leaching of recombinant Protein G.
**NOTE: the highest flow that beads withstand for 1 minute, without collapsing and the pressure reaching 1 MPa.
Usage:Reusable for up to 10 times without significant loss of binding capacity.
These beads are for use in column purification. If used in batch purification, we recommend not exceeding 150 x g when centrifuging.
Store beads at 4°C.
The beads may be damaged above 40°C.
DO NOT FREEZE.
Wash beads 3 times with 3x bead volume of desired buffer before use.
Applications:
- Purification of monoclonal and polyclonal antibodies from culture media, serum, ascites fluid or hybridoma supernatants.
- Isolation of antibody/antigen complexes in immunoprecipitation experiments, since only the Fc region is involved in antibody binding and the Fab region is available for binding antigen. -
说明
This product is manufactured by BioVision, an Abcam company and was previously called 6513 Hi-Bind™ Protein G-Agarose. 6513-5 is the same size as the 5 ml size of ab193258.
Protein G Agarose (High Affinity) beads are specially prepared for high IgG binding by covalently coupling recombinant Protein G to 6% cross-linked agarose beads, the most popular resin for protein affinity purification methods. Protein G is a genetically engineered protein containing three IgG-binding regions of native Protein G. The cell wall binding region, albumin binding region and other non-specific regions have been eliminated from the recombinant Protein G to ensure maximum specific IgG binding. The coupling technique is optimized to give a higher binding capacity for IgG and minimum leaching of recombinant Protein G than standard Protein G agarose beads. The IgG binding capacity of Protein G Agarose (High Affinity) is ≥ 30 mg of human or rabbit IgG per mL of wet beads. The Protein G Agarose (High Affinity) beads display high chemical and physical stability, as well as high flow rate, hydrophilicity and high gel strength.
-
经测试应用
适用于: IP, Purificationmore details
性能
-
存放说明
Store at +4°C. Please refer to protocols. -
组件 标识符 100 ml 25 ml 5 ml 1 ml Hi-Bind™ Protein G-Agarose — — — 1 x 1ml -
研究领域
相关产品
-
Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab193258于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IP |
Use at an assay dependent concentration.
Isolation of antibody/antigen complexes in immunoprecipitation experiments, since only the Fc region is involved in antibody binding and the Fab region is available for binding antigen. |
|
Purification |
Use at an assay dependent concentration.
Purification of monoclonal and polyclonal antibodies from culture media, serum, ascites fluid or hybridoma supernatants. |
说明 |
---|
IP
Use at an assay dependent concentration. Isolation of antibody/antigen complexes in immunoprecipitation experiments, since only the Fc region is involved in antibody binding and the Fab region is available for binding antigen. |
Purification
Use at an assay dependent concentration. Purification of monoclonal and polyclonal antibodies from culture media, serum, ascites fluid or hybridoma supernatants. |
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
Datasheet download
文献 (4)
ab193258 被引用在 4 文献中.
- Klucnika A et al. REC drives recombination to repair double-strand breaks in animal mtDNA. J Cell Biol 222:N/A (2023). PubMed: 36355348
- Ma Y miR-4735-3p inhibits cell migration and invasion of gastric cancer by downregulating NEDD9. Oncol Lett 24:253 (2022). PubMed: 35765282
- Mitchell AV et al. FOXQ1 recruits the MLL complex to activate transcription of EMT and promote breast cancer metastasis. Nat Commun 13:6548 (2022). PubMed: 36319643
- Goloshvili G et al. Sodium nitroprusside induces H-Ras depalmitoylation and alters the cellular response to hypoxia in differentiated and undifferentiated PC12 cells. Cell Biochem Funct 37:545-552 (2019). PubMed: 31429100