Anti-Protein C抗体[6B2] (ab36407)

概述

  • 产品名称Anti-Protein C抗体[6B2]
    参阅全部 Protein C 一抗
  • 描述
    小鼠单克隆抗体[6B2] to Protein C
  • 经测试应用适用于: ICC/IF, IHC-P, ELISA, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length native protein (purified) from human plasma.

性能

应用

Our Abpromise guarantee covers the use of ab36407 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
IHC-P Use at an assay dependent concentration.
ELISA 1/30000.
WB Use at an assay dependent concentration. Use under non reducing condition. Predicted molecular weight: 52 kDa.

靶标

  • 功能Protein C is a vitamin K-dependent serine protease that regulates blood coagulation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids (PubMed:25618265). Exerts a protective effect on the endothelial cell barrier function (PubMed:25651845).
  • 组织特异性Plasma; synthesized in the liver.
  • 疾病相关Thrombophilia due to protein C deficiency, autosomal dominant
    Thrombophilia due to protein C deficiency, autosomal recessive
  • 序列相似性Belongs to the peptidase S1 family.
    Contains 2 EGF-like domains.
    Contains 1 Gla (gamma-carboxy-glutamate) domain.
    Contains 1 peptidase S1 domain.
  • 翻译后修饰The vitamin K-dependent, enzymatic carboxylation of some Glu residues allows the modified protein to bind calcium.
    N- and O-glycosylated. Partial (70%) N-glycosylation of Asn-371 with an atypical N-X-C site produces a higher molecular weight form referred to as alpha. The lower molecular weight form, not N-glycosylated at Asn-371, is beta. O-glycosylated with core 1 or possibly core 8 glycans.
    The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
    May be phosphorylated on a Ser or Thr in a region (AA 25-30) of the propeptide.
  • 细胞定位Secreted. Golgi apparatus. Endoplasmic reticulum.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Activation peptide antibody
    • Anticoagulant protein C antibody
    • APC antibody
    • Autoprothrombin IIA antibody
    • Blood coagulation factor XIV antibody
    • EC 3.4.21.69 antibody
    • PC antibody
    • proC antibody
    • PROC_HUMAN antibody
    • PROC1 antibody
    • Protein C (inactivator of coagulation factors Va and VIIIa) antibody
    • THPH3 antibody
    • THPH4 antibody
    • Vitamin K dependent protein C antibody
    see all

Anti-Protein C antibody [6B2] 图像

  • Paraffin-embedded Human liver tissue immunostained with ab36407

  • ICC/IF image of ab36407 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab36407, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Protein C antibody [6B2] (ab36407)参考文献

ab36407 has not yet been referenced specifically in any publications.

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All of these antibodies are raised against full length Protein C. So we do not know what epitope(s) will be recognized by these antibodies.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"