Anti-Protein A抗体[SPA-27] (Biotin) (ab49734)

概述

  • 产品名称Anti-Protein A抗体[SPA-27] (Biotin)
    参阅全部 Protein A 一抗
  • 描述
    小鼠单克隆抗体[SPA-27] to Protein A (Biotin)
  • 偶联物Biotin
  • 特异性Specific for Protein A and shows no cross-reactivity with Protein G. Reacts with free Protein A or IgG bound Protein A and does not interfere with the Fc binding activity of Protein A. The activity of this antibody is due to specific antigen antibody interaction with protein A; Protein A reacts minimally with the Fc portion of this mouse IgG1 antibody. The antibody will bind to protein A even in the presence of IgG.
  • 经测试应用适用于: ELISA, Dot blotmore details
  • 种属反应性
    与反应: Staphylococcus aureus
  • 免疫原

    Purified Protein A from Cowan I strain of Staphy­lococcus aureus.

  • 常规说明

    The immunoglobulin fraction of the ascites fluid is conjugated to epsilon amino caproyl biotin.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 15mM Sodium Azide
    Constituents: 1% BSA, 0.01M PBS, pH 7.4
  • Concentration information loading...
  • 纯度IgG fraction
  • 纯化说明Immunoglobulin Fraction of Mouse Ascites Fluid
  • 克隆单克隆
  • 克隆编号SPA-27
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab49734 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/80000.
Dot blot 1/5000.

靶标

  • 相关性Protein A is a surface protein of S.aureus which binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro, and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology.
  • 细胞定位Secreted; cell wall.
  • 数据库链接
    • 别名
      • gG-binding protein A antibody
      • Immunoglobulin G binding protein A antibody
      • SPA antibody
      • Staphylococcal protein A antibody

    Anti-Protein A antibody [SPA-27] (Biotin) (ab49734)参考文献

    ab49734 has not yet been referenced specifically in any publications.

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