Anti-Protein A抗体(Biotin) (ab7246)

概述

  • 产品名称Anti-Protein A抗体(Biotin)
    参阅全部 Protein A 一抗
  • 描述
    山羊多克隆抗体to Protein A (Biotin)
  • 偶联物Biotin
  • 经测试应用适用于: Dot blot, ELISA, IHC-Fr, Immunomicroscopy, WBmore details
  • 免疫原

    Protein A [Staphylococcus aureus]

  • 常规说明

    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per goat IgG molecule.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 7.60
    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate
  • Concentration information loading...
  • 纯度IgG fraction
  • 纯化说明This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab7246 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Dot blot
ELISA
IHC-Fr
Immunomicroscopy
WB
  • 应用说明Immunoblotting, ELISA(1:4,000 to 1:20,000), immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
  • 靶标

    • 相关性Protein A is a surface protein of S.aureus which binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro, and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology.
    • 细胞定位Secreted; cell wall.
    • 别名
      • gG-binding protein A antibody
      • Immunoglobulin G binding protein A antibody
      • SPA antibody
      • Staphylococcal protein A antibody

    Anti-Protein A antibody (Biotin) (ab7246)参考文献

    ab7246 has not yet been referenced specifically in any publications.

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