Anti-Proteasome subunit beta type 4抗体(ab55628)


  • 产品名称Anti-Proteasome subunit beta type 4抗体
    参阅全部 Proteasome subunit beta type 4 一抗
  • 描述
    小鼠单克隆抗体to Proteasome subunit beta type 4
  • 经测试应用适用于: IHC-P, ICC/IF, WB, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein corresponding to Human Proteasome subunit beta type 4 aa 1-265.
    Database link: P28070


  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆单克隆
  • 同种型IgG1
  • 轻链类型kappa
  • 研究领域


Our Abpromise guarantee covers the use of ab55628 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 3 µg/ml.
ICC/IF 1/500.
WB Use a concentration of 1 - 5 µg/ml. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


  • 功能The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Mediates the lipopolysaccharide-induced signal macrophage proteasome (By similarity). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1.
  • 序列相似性Belongs to the peptidase T1B family.
  • 细胞定位Cytoplasm. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 26 kDa prosomal protein antibody
    • HN3 antibody
    • HsBPROS26 antibody
    • HSN3 antibody
    • Macropain beta chain antibody
    • Multicatalytic endopeptidase complex beta chain antibody
    • PROS-26 antibody
    • PROS26 antibody
    • Proteasome (prosome macropain) subunit beta type 4 antibody
    • Proteasome beta 4 subunit antibody
    • Proteasome beta chain antibody
    • Proteasome chain 3 antibody
    • Proteasome subunit beta type 4 antibody
    • Proteasome subunit beta type-4 antibody
    • Proteasome subunit HsN3 antibody
    • PSB4_HUMAN antibody
    • PSMB 4 antibody
    • PSMB4 antibody
    see all

Anti-Proteasome subunit beta type 4 antibody 图像

  • Proteasome subunit beta type 4 antibody (ab55628) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human testis.
  • Western blot against tagged recombinant protein immunogen using ab55628 Proteasome subunit beta type 4 antibody at 1ug/ml. Predicted band size of immunogen is 55 kDa
  • Overlay histogram showing HeLa cells stained with ab55628 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55628, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Proteasome subunit beta type 4 antibody (ab55628)参考文献

This product has been referenced in:
  • Thaker NG  et al. Identification of survival genes in human glioblastoma cells by small interfering RNA screening. Mol Pharmacol 76:1246-55 (2009). Read more (PubMed: 19783622) »
  • Catlow K  et al. Identification of a gastrin response element in the vesicular monoamine transporter type 2 promoter and requirement of 20 S proteasome subunits for transcriptional activity. J Biol Chem 282:17069-77 (2007). ICC/IF ; Human . Read more (PubMed: 17442673) »

See all 2 Publications for this product

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