重组
RabMAb

Anti-Proteasome subunit alpha type 6抗体[EPR5835] (ab109377)

概述

  • 产品名称
    Anti-Proteasome subunit alpha type 6抗体[EPR5835]
    参阅全部 Proteasome subunit alpha type 6 一抗
  • 描述
    兔单克隆抗体[EPR5835] to Proteasome subunit alpha type 6
  • 经测试应用
    适用于: WB, IHC-P, ICC, Flow Cytmore details
    不适用于: IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Proteasome 20S alpha 6 aa 200-300 (C terminal).
    Database link: P60900

  • 阳性对照
    • WB: HepG2, 293T, HeLa and Jurkat cell lysates IHC-P: Human colon tissue
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

应用

Our Abpromise guarantee covers the use of ab109377 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/50000 - 1/200000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 °C, below boiling, and then let cool for 10-20 minutes.
ICC 1/250 - 1/500.
Flow Cyt 1/100 - 1/500. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 应用说明
    Is unsuitable for IP.
  • 靶标

    • 功能
      The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.
    • 序列相似性
      Belongs to the peptidase T1A family.
    • 细胞定位
      Cytoplasm. Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 27 kDa prosomal protein antibody
      • IOTA antibody
      • Macropain iota chain antibody
      • Macropain subunit iota antibody
      • MGC22756 antibody
      • MGC2333 antibody
      • MGC23846 antibody
      • Multicatalytic endopeptidase complex iota chain antibody
      • p27K antibody
      • PROS 27 antibody
      • PROS-27 antibody
      • PROS27 antibody
      • Prosomal P27K protein antibody
      • Proteasome (prosome macropain) subunit alpha type 6 antibody
      • Proteasome iota chain antibody
      • Proteasome subunit alpha type 6 antibody
      • Proteasome subunit alpha type-6 antibody
      • Proteasome subunit iota antibody
      • PSA6_HUMAN antibody
      • PSMA 6 antibody
      • PSMA6 antibody
      see all

    图片

    • All lanes : Anti-Proteasome subunit alpha type 6 antibody [EPR5835] (ab109377) at 1/50000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 27 kDa
      Observed band size : 27 kDa
    • ab109377, at a 1/250 dilution, staining Proteasome 20S alpha 6 in paraffin embedded Human colon tissue by Immunohistochemistry.

    文献

    This product has been referenced in:
    • Zhu Y  et al. Identification of biomarker microRNAs for predicting the response of colorectal cancer to neoadjuvant chemoradiotherapy based on microRNA regulatory network. Oncotarget 8:2233-2248 (2017). WB ; Human . Read more (PubMed: 27903980) »
    • Ando R  et al. Decreased proteasomal activity causes photoreceptor degeneration in mice. Invest Ophthalmol Vis Sci 55:4682-90 (2014). Mouse . Read more (PubMed: 24994871) »

    See all 2 Publications for this product

    客户评价及客户问答

    There are currently no Customer reviews or Questions for ab109377.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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