兔多克隆抗体to Proteasome subunit alpha type 6
Mouse, Rat, Pig, Zebrafish
Recombinant fragment corresponding to Human Proteasome subunit alpha type 6 aa 57-244.
Database link: P60900
WB: 293T, A431, H1299, HeLa, HepG2 and MOLT4 cell lysates
IF: A431 cells
IHC-P: H1299 xenograft
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 27 kDa.
1/100 - 1/500.
1/100 - 1/200.
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.
Belongs to the peptidase T1A family.
Information by UniProt
27 kDa prosomal protein antibody
Macropain iota chain antibody
Western blot - Anti-Proteasome subunit alpha type 6 antibody (ab97563)
All lanes : Anti-Proteasome subunit alpha type 6 antibody (ab97563) at 1/1000 dilution Lane 1 : 293T whole cell lysate Lane 2 : A431 whole cell lysate Lane 3 : H1299 whole cell lysate Lane 4 : HeLa whole cell lysate Lysates/proteins at 30 µg per lane. Predicted band size: 27 kDa 12% SDS PAGE
Immunocytochemistry/ Immunofluorescence - Anti-Proteasome subunit alpha type 6 antibody (ab97563)
ab97563, at a 1/200 dilution, staining Proteasome subunit alpha type 6 in paraformaldehyde-fixed A431 cells by Immunofluorescence analysis. Right image is merged with a DNA probe.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome subunit alpha type 6 antibody (ab97563)
ab97563, at a 1/100 dilution, staining Proteasome subunit alpha type 6 in paraffin-embedded H1299 xenograft by Immunohistochemical analysis.
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