Anti-Proteasome subunit alpha type 6抗体(ab97563)

概述

  • 产品名称
    Anti-Proteasome subunit alpha type 6抗体
    参阅全部 Proteasome subunit alpha type 6 一抗
  • 描述
    兔多克隆抗体to Proteasome subunit alpha type 6
  • 经测试应用
    适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Pig, Zebrafish
  • 免疫原

    Recombinant fragment corresponding to Human Proteasome subunit alpha type 6 aa 57-244.
    Database link: P60900

  • 阳性对照
    • WB: 293T, A431, H1299, HeLa, HepG2 and MOLT4 cell lysates IF: A431 cells IHC-P: H1299 xenograft

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 20% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab97563 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 27 kDa.
IHC-P 1/100 - 1/500.
ICC/IF 1/100 - 1/200.

靶标

  • 功能
    The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.
  • 序列相似性
    Belongs to the peptidase T1A family.
  • 细胞定位
    Cytoplasm. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 27 kDa prosomal protein antibody
    • IOTA antibody
    • Macropain iota chain antibody
    • Macropain subunit iota antibody
    • MGC22756 antibody
    • MGC2333 antibody
    • MGC23846 antibody
    • Multicatalytic endopeptidase complex iota chain antibody
    • p27K antibody
    • PROS 27 antibody
    • PROS-27 antibody
    • PROS27 antibody
    • Prosomal P27K protein antibody
    • Proteasome (prosome macropain) subunit alpha type 6 antibody
    • Proteasome iota chain antibody
    • Proteasome subunit alpha type 6 antibody
    • Proteasome subunit alpha type-6 antibody
    • Proteasome subunit iota antibody
    • PSA6_HUMAN antibody
    • PSMA 6 antibody
    • PSMA6 antibody
    see all

图片

  • All lanes : Anti-Proteasome subunit alpha type 6 antibody (ab97563) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate
    Lane 3 : H1299 whole cell lysate
    Lane 4 : HeLa whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 27 kDa
  • ab97563, at a 1/200 dilution, staining Proteasome subunit alpha type 6 in paraformaldehyde-fixed A431 cells by Immunofluorescence analysis. Right image is merged with a DNA probe.
  • ab97563, at a 1/100 dilution, staining Proteasome subunit alpha type 6 in paraffin-embedded H1299 xenograft by Immunohistochemical analysis.

文献

This product has been referenced in:
  • Piccinini AM  et al. Distinct microenvironmental cues stimulate divergent TLR4-mediated signaling pathways in macrophages. Sci Signal 9:ra86 (2016). WB . Read more (PubMed: 27577261) »

See 1 Publication for this product

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