Anti-Proteasome Inhibitor subunit 1 PI3 1抗体(ab3893)

概述

  • 产品名称
    Anti-Proteasome Inhibitor subunit 1 PI3 1抗体
    参阅全部 Proteasome Inhibitor subunit 1 PI3 1 一抗
  • 描述
    山羊多克隆抗体to Proteasome Inhibitor subunit 1 PI3 1
  • 特异性
    This antibody is expected to recognise isoform 1 (NP_006805, NP_848693) only, not isoform 2 (NP_848694).
  • 经测试应用
    适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide: DHLPPPGYDDMYL, corresponding to C terminal amino acids 259-271 of Human Proteasome Inhibitor subunit 1 PI3 1.

  • 阳性对照
    • Human Kidney lysate.
  • 常规说明
    Principal Names – PSMF1; PI31; proteasome (prosome, macropain) inhibitor subunit 1 (PI31); proteasome inhibitor hP131 subunit Official Gene Symbol - PSMF1 GenBank Accession Number – NP_006805; NP_848693 LocusLink ID - 9491 (human); 228769 (mouse).

性能

应用

Our Abpromise guarantee covers the use of ab3893 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
WB
  • 应用说明
    ELISA: 1/32000.
    WB: Use at a concentration of 1 - 3 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    图片

    • ab3893 staining (1µg/ml) of Human Kidney lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

    实验方案

    文献

    ab3893 has not yet been referenced specifically in any publications.

    客户评价及客户问答

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (MEF)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    15 µg
    Specification
    MEF
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 May 17 2017

    Thank you for sending the details of your protocol and the picture of your blot. I really think that the background bands are due to the secondary. What appears to be happening is that the secondary is binding additional proteins and reacting with the ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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