Anti-Proteasome 26S S10抗体(ab58116)

概述

  • 产品名称Anti-Proteasome 26S S10抗体
    参阅全部 Proteasome 26S S10 一抗
  • 描述
    小鼠单克隆抗体to Proteasome 26S S10
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment: EGGANPDAKD HYEATAMHRA AAKGNLKMIH ILLYYKASTN IQDTEGNTPL HLACDEERVE EAKLLVSQGA SIYIENKEEK TPLQVAKGGL GLILKRMVEG , corresponding to amino acids 127-226 of Human Proteasome 26S S10

性能

应用

Our Abpromise guarantee covers the use of ab58116 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 24 kDa.
IHC-P Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 10 µg/ml.

靶标

  • 功能Acts as a chaperone during the assembly of the 26S proteasome, specifically of the PA700/19S regulatory complex (RC). In the initial step of the base subcomplex assembly is part of an intermediate PSMD10:PSMC4:PSMC5:PAAF1 module which probably assembles with a PSMD5:PSMC2:PSMC1:PSMD2 module.
    Acts as an proto-oncoprotein by being involved in negative regulation of tumor suppressors RB1 and p53/TP53. Overexpression is leading to phosphorylation of RB1 and proteasomal degradation of RB1. Regulates CDK4-mediated phosphorylation of RB1 by competing with CDKN2A for binding with CDK4. Facilitates binding of MDM2 to p53/TP53 and the mono- and polyubiquitination of p53/TP53 by MDM2 suggesting a function in targeting the TP53:MDM2 complex to the 26S proteasome. Involved in p53-independent apoptosis. Involved in regulation of NF-kappa-B by retaining it in the cytoplasm. Binds to the NF-kappa-B component RELA and accelerates its XPO1/CRM1-mediated nuclear export.
  • 组织特异性Overexpressed in hepatocellular carcinomas.
  • 序列相似性Contains 7 ANK repeats.
  • 细胞定位Cytoplasm. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 26S proteasome non-ATPase regulatory subunit 10 antibody
    • 26S proteasome regulatory subunit p28 antibody
    • Ankyrin repeat protein antibody
    • dJ889N15.2 antibody
    • Gankyrin antibody
    • Hepatocellular carcinoma-associated protein p28 II antibody
    • p28 antibody
    • p28(GANK) antibody
    • Proteasome (prosome, macropain) 26S subunit, non ATPase, 10 antibody
    • PSD10_HUMAN antibody
    • Psmd10 antibody
    see all

Anti-Proteasome 26S S10 antibody 图像

  • Proteasome 26S S10 antibody (ab58116) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human tonsil.


  • Predicted band size : 24 kDa
    Proteasome 26S S10 antibody (ab58116) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • ab58116 at 10 ug/ml staining Proteasome 20S S10 in human Hella cells by Immunocytochemistry/ Immunofluorescence.

Anti-Proteasome 26S S10 antibody (ab58116)参考文献

ab58116 has not yet been referenced specifically in any publications.

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