Anti-Proteasome 20S LMP7抗体(ab58094)


  • 产品名称Anti-Proteasome 20S LMP7抗体
    参阅全部 Proteasome 20S LMP7 一抗
  • 描述
    小鼠单克隆抗体to Proteasome 20S LMP7
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment corresponding to Human Proteasome 20S LMP7 aa 173-273.


    Database link: P28062


  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆单克隆
  • 同种型IgG2a
  • 轻链类型kappa
  • 研究领域


Our Abpromise guarantee covers the use of ab58094 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml.
IHC-P Use a concentration of 3 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.


  • 功能The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB5 by PSMB8 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues. Acts as a major component of interferon gamma-induced sensitivity. Plays a key role in apoptosis via the degradation of the apoptotic inhibitor MCL1. May be involved in the inflammatory response pathway. In cancer cells, substitution of isoform 1 (E2) by isoform 2 (E1) results in immunoproteasome deficiency.
  • 疾病相关Defects in PSMB8 are the cause of JMP syndrome (JMPS) [MIM:613732]; also called joint contractures muscular atrophy microcytic anemia and panniculitis-induced lipodystrophy. JBTS1 is an autoinflammatory disorder characterized by childhood onset of joint stiffness and severe contractures of the hands and feet, erythematous skin lesions with subsequent development of severe lipodystrophy, and laboratory evidence of immune dysregulation. Accompanying features include muscle weakness and atrophy, hepatosplenomegaly, and microcytic anemia.
  • 序列相似性Belongs to the peptidase T1B family.
  • 发展阶段Highly expressed in immature dendritic cells (at protein level).
  • 翻译后修饰Autocleaved. The resulting N-terminal Thr residue of the mature subunit is responsible for the nucleophile proteolytic activity.
  • 细胞定位Cytoplasm. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ALDD antibody
    • D6S216 antibody
    • D6S216E antibody
    • Large multifunctional peptidase 7 antibody
    • Large multifunctional protease 7 antibody
    • LMP 7 antibody
    • LMP7 antibody
    • Low molecular mass protein 7 antibody
    • Low molecular weight protein 7 antibody
    • Macropain subunit C13 antibody
    • MGC1491 antibody
    • Multicatalytic endopeptidase complex subunit C13 antibody
    • NKJO antibody
    • OTTHUMP00000062981 antibody
    • Protease component C13 antibody
    • Proteasome (prosome macropain) subunit beta type 8 antibody
    • Proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctional peptidase 7) antibody
    • Proteasome beta 8 subunit antibody
    • Proteasome catalytic subunit 3i antibody
    • Proteasome component C13 antibody
    • Proteasome related gene 7 antibody
    • Proteasome subunit beta 5i antibody
    • Proteasome subunit beta 8 antibody
    • Proteasome subunit beta type 8 antibody
    • Proteasome subunit beta type antibody
    • Proteasome subunit beta type-8 antibody
    • Proteasome subunit beta-5i antibody
    • Proteasome subunit Y2 antibody
    • PSB8_HUMAN antibody
    • PSMB 8 antibody
    • PSMB5i antibody
    • PSMB8 antibody
    • Really interesting new gene 10 protein antibody
    • RING 10 antibody
    • RING10 antibody
    • Y2 antibody
    see all

Anti-Proteasome 20S LMP7 antibody 图像

  • Proteasome 20S LMP7 antibody (ab58094) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human colon.
  • Western blot against tagged recombinant protein immunogen using ab58094 Proteasome 20S LMP7 antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa
  • ab58094 at 10 ug/ml staining Proteasome 20S LMP7 in human Hella cells by Immunocytochemistry/ Immunofluorescence.

Anti-Proteasome 20S LMP7 antibody (ab58094)参考文献

This product has been referenced in:
  • Arellano-Garcia ME  et al. Interferon-? induces immunoproteasomes and the presentation of MHC I-associated peptides on human salivary gland cells. PLoS One 9:e102878 (2014). WB ; Human . Read more (PubMed: 25102056) »
  • Fellerhoff B  et al. The LMP7-K Allele of the Immunoproteasome Exhibits Reduced Transcript Stability and Predicts High Risk of Colon Cancer. Cancer Res 71:7145-54 (2011). WB ; Human . Read more (PubMed: 22037870) »

See all 2 Publications for this product

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (4-15%)
Sample Human Cell lysate - whole cell (Lymphoma)
Specification Lymphoma
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. satyendra tripathi

Verified customer

提交于 Oct 07 2014