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Our Abpromise guarantee covers the use of ab3328 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Can be blocked with Proteasome 20S LMP2 peptide (ab4944). (worked at 1/2000 (PMID 17114438))|
|IHC-P||Use a concentration of 1 µg/ml.|
Western blot of proteasome 20S LMP2 in 50 ug mouse embryo fibroblast cell extract using ab3328.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue (neurons in HIV encephalitis) sections labelling LMP2 with ab3328 at 1/1000. Immunofluorescence staining for LMP2 shows colocalization with the neuronal marker NeuN. LMP2 is present in granular deposits in the parikaryon. Scale bar = 10 μm.