兔单克隆抗体[EPR5451] to Proteasome 20S C2
Mouse, Rat, Human
Synthetic peptide within Human Proteasome 20S C2 aa 1-100. The exact sequence is proprietary.
Database link: P25786
HeLa, Jurkat, HepG2, and PC-3 cell lysates
Human breast tissue
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This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Predicted molecular weight: 30 kDa.
1/10 - 1/100.
1/250 - 1/500. Perform antigen retrieval
1/100 - 1/250.
Is unsuitable for Flow Cyt.
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Mediates the lipopolysaccharide-induced signal transduction in the macrophage proteasome (By similarity). Might be involved in the anti-inflammatory response of macrophages during the interaction with C.albicans heat-inactivated cells.
Belongs to the peptidase T1A family.
Information by UniProt
30 kDa prosomal protein antibody
HC 2 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome 20S C2 antibody [EPR5451] (ab109500)
Immunohistochemical analysis of paraffin-embedded Human breast tissue using ab109500 at 1/250 dilution.
Western blot - Anti-Proteasome 20S C2 antibody [EPR5451] (ab109500)
All lanes : Anti-Proteasome 20S C2 antibody [EPR5451] (ab109500) at 1/1000 dilution Lane 1 : HeLa cell lysate Lane 2 : Jurkat cell lysate Lane 3 : HepG2 cell lysate Lane 4 : PC-3 cell lysate Lysates/proteins at 10 µg per lane. Secondary All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution Predicted band size: 30 kDa
has not yet been referenced specifically in any publications.
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