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Tissue/ cell preparation: Proteasomal preparation isolated from human red blood cells.
Our Abpromise guarantee covers the use of ab22673 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent concentration. PubMed: 23840376|
|IHC-Fr||Use at an assay dependent concentration.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 25 - 30 kDa.|
|IP||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration. See Abreview.|
ab22673 staining human Hela cells by ICC/IF. Cells were formaldehyde fixed, permeabilized in 0.1% Triton and blocked in 3% BSA for 15 minutes at room temperature. The primary antibody was diluted 1/2000 (3% BSA) and incubated with sample for 1 hour. An Alexa Fluor® 488 conjugated goat polyclonal to rabbit, diluted 1/1000 was used as secondary.
This image is courtesy of an anonymous abreview.Blocked with 5% milk for 30 minutes at 27°C.