RabMAb

Anti-Proteasome 20S alpha 5抗体[EPR5833] (ab109533)

概述

  • 产品名称Anti-Proteasome 20S alpha 5抗体[EPR5833]
    参阅全部 Proteasome 20S alpha 5 一抗
  • 描述
    兔单克隆抗体[EPR5833] to Proteasome 20S alpha 5
  • 经测试应用适用于: WB, IHC-P, Flow Cytmore details
    不适用于: ICC or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Proteasome 20S alpha 5 aa 200-300 (C terminal).
    Database link: P28066

  • 阳性对照
    • HepG2, Hela, 293T and JAR cell lysates; Human brain tissue.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab109533 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/50000. Predicted molecular weight: 26 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 应用说明Is unsuitable for ICC or IP.
  • 靶标

    • 功能The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.
    • 组织特异性Expressed in fetal brain (at protein level).
    • 序列相似性Belongs to the peptidase T1A family.
    • 细胞定位Cytoplasm. Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Macropain zeta chain antibody
      • Multicatalytic endopeptidase complex zeta chain antibody
      • Proteasome (prosome macropain) subunit alpha type 5 antibody
      • Proteasome alpha5 subunit antibody
      • Proteasome component 5 antibody
      • Proteasome subunit alpha type 5 antibody
      • Proteasome subunit alpha type-5 antibody
      • Proteasome subunit zeta antibody
      • Proteasome zeta chain antibody
      • PSA5_HUMAN antibody
      • PSC5 antibody
      • PSMA5 antibody
      • ZETA antibody
      see all

    Anti-Proteasome 20S alpha 5 antibody [EPR5833] 图像

    • All lanes : Anti-Proteasome 20S alpha 5 antibody [EPR5833] (ab109533) at 1/10000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : Hela cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : JAR cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 26 kDa
    • ab109533, at 1/100 dilution, staining Proteasome 20S alpha 5 in paraffin embedded Human brain tissue by Immunohistochemistry.

    Anti-Proteasome 20S alpha 5 antibody [EPR5833] (ab109533)参考文献

    ab109533 has not yet been referenced specifically in any publications.

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