兔单克隆抗体[EPR5833] to Proteasome 20S alpha 5
Mouse, Rat, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Proteasome 20S alpha 5 aa 200-300 (C terminal).
Database link: P28066
HepG2, Hela, 293T and JAR cell lysates; Human brain tissue.
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This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10000 - 1/50000. Predicted molecular weight: 26 kDa.
1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Is unsuitable for ICC or IP.
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.
Expressed in fetal brain (at protein level).
Belongs to the peptidase T1A family.
Information by UniProt
Macropain zeta chain antibody
Multicatalytic endopeptidase complex zeta chain antibody
Proteasome (prosome macropain) subunit alpha type 5 antibody
Western blot - Proteasome 20S alpha 5 antibody [EPR5833] (ab109533)
All lanes : Anti-Proteasome 20S alpha 5 antibody [EPR5833] (ab109533) at 1/10000 dilution Lane 1 : HepG2 cell lysate Lane 2 : Hela cell lysate Lane 3 : 293T cell lysate Lane 4 : JAR cell lysate Lysates/proteins at 10 µg per lane. Predicted band size: 26 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Proteasome 20S alpha 5 antibody [EPR5833] (ab109533)
ab109533, at 1/100 dilution, staining Proteasome 20S alpha 5 in paraffin embedded Human brain tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
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