Anti-Proteasome 20S alpha 5抗体(ab11437)

概述

  • 产品名称
    Anti-Proteasome 20S alpha 5抗体
    参阅全部 Proteasome 20S alpha 5 一抗
  • 描述
    兔多克隆抗体to Proteasome 20S alpha 5
  • 经测试应用
    适用于: IHC-P, ICC/IF, ICC, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Hamster, Human
    预测可用于: Cow, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to Human Proteasome 20S alpha 5 aa 120-133.
    Sequence:

    ALQFGEEDADPGAM


    Database link: P28066
    (Peptide available as ab41791)

  • 阳性对照
    • Hela cell extract/cell line slide.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.05% Sodium azide
    Constituents: 99% PBS, 0.1% BSA
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab11437 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 µg/ml.
ICC Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 26.5 kDa (predicted molecular weight: 26.5 kDa).Can be blocked with Human Proteasome 20S alpha 5 peptide (ab41791).

靶标

图片

  • All lanes : Anti-Proteasome 20S alpha 5 antibody (ab11437)

    Lane 1 : HeLa cell extract.
    Lane 2 : HeLa cell extract with blocking peptide.


    Predicted band size : 26.5 kDa
    Observed band size : 26.5 kDa
  • Immunofluorescence of HeLa cells using ab11437.
  • IHC image of ab11437 staining in human liver carcinoma liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab11437, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

文献

This product has been referenced in:
  • Mori F  et al. Multitargeting activity of miR-24 inhibits long-term melatonin anticancer effects. Oncotarget 7:20532-48 (2016). WB . Read more (PubMed: 26967561) »
  • Freudenburg W  et al. Reduction in ATP levels triggers immunoproteasome activation by the 11S (PA28) regulator during early antiviral response mediated by IFNß in mouse pancreatic ß-cells. PLoS One 8:e52408 (2013). Mouse . Read more (PubMed: 23383295) »

See all 4 Publications for this product

客户评价及客户问答

Thank you for contacting us for technical support with ab11437, I'm sorry to hear you are experiencing problems with this antibody. Could you please clarify the type of cells in culture that you used? We have not tested ab11437 on whole sections, on...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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