Anti-Proteasome 20S alpha 3 (phospho S250)抗体(ab79184)

概述

  • 产品名称
    Anti-Proteasome 20S alpha 3 (phospho S250)抗体
    参阅全部 Proteasome 20S alpha 3 一抗
  • 描述
    兔多克隆抗体to Proteasome 20S alpha 3 (phospho S250)
  • 特异性
    Detects endogenous levels of Proteasome 20S alpha 3 only when phosphorylated at serine 250.
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Synthetic peptide corresponding to Human Proteasome 20S alpha 3 (phospho S250).
    Database link: P25788

  • 阳性对照
    • Extracts from 3T3 cells, treated with EGF (200 ng/ml, 30 minutes)

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    Affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab79184 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 28 kDa.
ELISA 1/5000.

靶标

  • 功能
    The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Binds to the C-terminus of CDKN1A and thereby mediates its degradation. Negatively regulates the membrane trafficking of the cell-surface thromboxane A2 receptor (TBXA2R) isoform 2.
  • 序列相似性
    Belongs to the peptidase T1A family.
  • 细胞定位
    Cytoplasm. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • HC8 antibody
    • Macropain subunit C8 antibody
    • MGC12306 antibody
    • MGC32631 antibody
    • Multicatalytic endopeptidase complex subunit C8 antibody
    • Proteasome (prosome macropain) subunit alpha type 3 antibody
    • Proteasome alpha 3 subunit antibody
    • Proteasome component C8 antibody
    • Proteasome subunit alpha type 3 antibody
    • Proteasome subunit alpha type-3 antibody
    • Proteasome subunit C8 antibody
    • PSA3_HUMAN antibody
    • PSC8 antibody
    • psmA3 antibody
    see all

图片

  • All lanes : Anti-Proteasome 20S alpha 3 (phospho S250) antibody (ab79184) at 1/500 dilution

    Lane 1 : Extracts from 3T3 cells, treated with EGF (200 ng/ml, 30 minutes)
    Lane 2 : Extracts from 3T3 cells, treated with EGF (200 ng/ml, 30 minutes) with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 28 kDa
    Observed band size : 28 kDa

实验方案

文献

ab79184 has not yet been referenced specifically in any publications.

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