RabMAb

Anti-Proteasome 20S alpha 3抗体[EPR5455] (ab109532)

概述

  • 产品名称Anti-Proteasome 20S alpha 3抗体[EPR5455]
    参阅全部 Proteasome 20S alpha 3 一抗
  • 描述
    兔单克隆抗体[EPR5455] to Proteasome 20S alpha 3
  • 经测试应用适用于: WB, IP, IHC-P, ICC/IFmore details
    不适用于: Flow Cyt
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Proteasome 20S alpha 3 aa 50-150.
    Database link: P25788

  • 阳性对照
    • A549, JAR, PC12 and NIH3T3 cell lysates; Human brain tissue; HeLa cells.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab109532 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 28 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • 应用说明Is unsuitable for Flow Cyt.
  • 靶标

    • 功能The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Binds to the C-terminus of CDKN1A and thereby mediates its degradation. Negatively regulates the membrane trafficking of the cell-surface thromboxane A2 receptor (TBXA2R) isoform 2.
    • 序列相似性Belongs to the peptidase T1A family.
    • 细胞定位Cytoplasm. Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • HC8 antibody
      • Macropain subunit C8 antibody
      • MGC12306 antibody
      • MGC32631 antibody
      • Multicatalytic endopeptidase complex subunit C8 antibody
      • Proteasome (prosome macropain) subunit alpha type 3 antibody
      • Proteasome alpha 3 subunit antibody
      • Proteasome component C8 antibody
      • Proteasome subunit alpha type 3 antibody
      • Proteasome subunit alpha type-3 antibody
      • Proteasome subunit C8 antibody
      • PSA3_HUMAN antibody
      • PSC8 antibody
      • psmA3 antibody
      see all

    Anti-Proteasome 20S alpha 3 antibody [EPR5455] 图像

    • All lanes : Anti-Proteasome 20S alpha 3 antibody [EPR5455] (ab109532) at 1/1000 dilution

      Lane 1 : A549 cell lysate
      Lane 2 : JAR cell lysate
      Lane 3 : PC12 cell lysate
      Lane 4 : NIH3T3 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 28 kDa
    • ab109532, at 1/100 dilution, staining Proteasome 20S alpha 3 in paraffin embedded Human brain tissue by Immunohistochemistry.
    • ab109532, at 1/100 dilution, staining Proteasome 20S alpha 3 in HeLa cells by Immunofluorescence.

    Anti-Proteasome 20S alpha 3 antibody [EPR5455] (ab109532)参考文献

    ab109532 has not yet been referenced specifically in any publications.

    Product Wall

    We do not have any data suggesting that the antibody has higher affinity for nuclear PSMA3 than for cytoplasmic PSMA3. The differences in staining intensity may be due to unequal distribution of the protein (more PSMA3 in the nucleus than in the cytopl...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"