RabMAb

Anti-Proteasome 20S alpha 2抗体[EPR5453] (ab109525)

概述

  • 产品名称Anti-Proteasome 20S alpha 2抗体[EPR5453]
    参阅全部 Proteasome 20S alpha 2 一抗
  • 描述
    兔单克隆抗体[EPR5453] to Proteasome 20S alpha 2
  • 经测试应用适用于: WB, IHC-P, Flow Cyt, ICC/IFmore details
    不适用于: IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Proteasome 20S alpha 2 aa 50-150.
    Database link: P25787

  • 阳性对照
    • HepG2, 293T, HeLa or JAR cell lysate; Human brain tissue; HeLa cells
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab109525 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 25 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes)
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/50 - 1/100.
  • 应用说明Is unsuitable for IP.
  • 靶标

    • 功能The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. PSMA2 may have a potential regulatory effect on another component(s) of the proteasome complex through tyrosine phosphorylation.
    • 序列相似性Belongs to the peptidase T1A family.
    • 翻译后修饰Phosphorylated on tyrosine residues; which may be important for nuclear import.
    • 细胞定位Cytoplasm. Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • HC3 antibody
      • Macropain subunit C3 antibody
      • MU antibody
      • Multicatalytic endopeptidase complex subunit C3 antibody
      • PMSA2 antibody
      • Proteasome (prosome macropain) subunit alpha type 2 antibody
      • Proteasome alpha 2 subunit antibody
      • Proteasome component C3 antibody
      • Proteasome subunit alpha type 2 antibody
      • Proteasome subunit alpha type-2 antibody
      • Proteasome subunit HC3 antibody
      • PSA2_HUMAN antibody
      • PSC2 antibody
      • PSC3 antibody
      • PSMA 2 antibody
      • psmA2 antibody
      see all

    Anti-Proteasome 20S alpha 2 antibody [EPR5453] 图像

    • All lanes : Anti-Proteasome 20S alpha 2 antibody [EPR5453] (ab109525) at 1/10000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : JAR cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

      Predicted band size : 25 kDa
    • ab109525, at a 1/250 dilution, staining Human Proteasome 20S alpha 2 in brain tissue, using Immunohistochemistry, (Formalin/PFA-fixed paraffin-embedded).
    • ab109525, at 1/50 dilution, staining Proteasome 20S alpha 2 in HeLa cells by Immunofluorescence.

    Anti-Proteasome 20S alpha 2 antibody [EPR5453] (ab109525)参考文献

    This product has been referenced in:
    • Pla A  et al. TLR4 mediates the impairment of ubiquitin-proteasome and autophagy-lysosome pathways induced by ethanol treatment in brain. Cell Death Dis 5:e1066 (2014). WB ; Mouse . Read more (PubMed: 24556681) »

    See 1 Publication for this product

    Product Wall

    Application Western blot
    Loading amount 30 µg
    Gel Running Conditions Reduced Denaturing (12 %)
    Sample Human Cell lysate - whole cell (HaCaT human immortalized keratinocyte cell line, 3)
    Specification HaCaT human immortalized keratinocyte cell line, 3
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Iva Doleckova

    Verified customer

    提交于 May 31 2014

    Thank you for your inquiry. Regarding ab109525, we have not actually measured non-IgG protein concentration. The only known non-IgG protein in our antibody is BSA (0.05%). Please contact us for the specific IgG concentration of the lot you received...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"