重组Anti-Proteasome 20S alpha 2/HC3抗体[EPR5453] (ab109525)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5453] to Proteasome 20S alpha 2/HC3
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Proteasome 20S alpha 2/HC3抗体[EPR5453]
参阅全部 Proteasome 20S alpha 2/HC3 一抗 -
描述
兔单克隆抗体[EPR5453] to Proteasome 20S alpha 2/HC3 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details
不适用于: IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- HepG2, 293T, HeLa or JAR cell lysate; Human brain tissue; HeLa cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5453 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109525于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 25 kDa.
|
IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
(Heat to 98°C, allow to cool for 10-20 minutes) |
|
ICC/IF |
1/50 - 1/100.
|
说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 25 kDa. |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes) |
ICC/IF
1/50 - 1/100. |
靶标
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功能
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. PSMA2 may have a potential regulatory effect on another component(s) of the proteasome complex through tyrosine phosphorylation. -
序列相似性
Belongs to the peptidase T1A family. -
翻译后修饰
Phosphorylated on tyrosine residues; which may be important for nuclear import. -
细胞定位
Cytoplasm. Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 5683 Human
- Entrez Gene: 19166 Mouse
- Entrez Gene: 29669 Rat
- Omim: 176842 Human
- SwissProt: P25787 Human
- SwissProt: P49722 Mouse
- SwissProt: P17220 Rat
- Unigene: 333786 Human
see all -
别名
- HC3 antibody
- Macropain subunit C3 antibody
- MU antibody
see all
图片
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All lanes : Anti-Proteasome 20S alpha 2/HC3 antibody [EPR5453] (ab109525) at 1/10000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : JAR cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution
Predicted band size: 25 kDa -
ab109525, at a 1/250 dilution, staining Human Proteasome 20S alpha 2/HC3 in brain tissue, using Immunohistochemistry, (Formalin/PFA-fixed paraffin-embedded).
Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
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ab109525, at 1/50 dilution, staining Proteasome 20S alpha 2/HC3 in HeLa cells by Immunofluorescence.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab109525 被引用在 4 文献中.
- Ni HM et al. Receptor-Interacting Serine/Threonine-Protein Kinase 3 (RIPK3)-Mixed Lineage Kinase Domain-Like Protein (MLKL)-Mediated Necroptosis Contributes to Ischemia-Reperfusion Injury of Steatotic Livers. Am J Pathol N/A:N/A (2019). PubMed: 31026418
- Alber AB et al. Single Live Cell Monitoring of Protein Turnover Reveals Intercellular Variability and Cell-Cycle Dependence of Degradation Rates. Mol Cell 71:1079-1091.e9 (2018). PubMed: 30146318
- Wang S et al. Increased hepatic receptor interacting protein kinase 3 expression due to impaired proteasomal functions contributes to alcohol-induced steatosis and liver injury. Oncotarget 7:17681-98 (2016). PubMed: 26769846
- Pla A et al. TLR4 mediates the impairment of ubiquitin-proteasome and autophagy-lysosome pathways induced by ethanol treatment in brain. Cell Death Dis 5:e1066 (2014). WB ; Mouse . PubMed: 24556681