Anti-Proteasome 19S S5A抗体[AH1.1] (ab20239)

概述

  • 产品名称Anti-Proteasome 19S S5A抗体[AH1.1]
    参阅全部 Proteasome 19S S5A 一抗
  • 描述
    小鼠单克隆抗体[AH1.1] to Proteasome 19S S5A
  • 经测试应用适用于: Flow Cyt, ICC/IF, IHC-Fr, IP, WBmore details
  • 种属反应性
    与反应: Human, Pig, Xenopus laevis
    不与反应: Mouse
  • 免疫原

    Sub unit 5a.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • 纯度IgG fraction
  • 克隆单克隆
  • 克隆编号AH1.1
  • 骨髓瘤Sp2/0-Ag14
  • 同种型IgG1
  • 轻链类型unknown
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab20239 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF 1/200.
IHC-Fr 1/100. Fix with acetone.
IP Use a concentration of 5 µg/ml.
WB Use at an assay dependent concentration. Predicted molecular weight: 41 kDa.

靶标

  • 功能Binds and presumably selects ubiquitin-conjugates for destruction. Displays selectivity for longer polyubiquitin chains. Modulates intestinal fluid secretion.
  • 序列相似性Belongs to the proteasome subunit S5A family.
    Contains 2 UIM (ubiquitin-interacting motif) repeats.
    Contains 1 VWFA domain.
  • 结构域The 2 UIM motifs are involved in the binding to a multi-ubiquitin chain in a cooperative way.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 26S protease subunit S5a antibody
    • 26S proteasome non ATPase regulatory subunit 4 antibody
    • 26S proteasome non-ATPase regulatory subunit 4 antibody
    • 26S proteasome regulatory subunit rpn10 antibody
    • 26S proteasome regulatory subunit S5A antibody
    • AF 1 antibody
    • AF antibody
    • AF1 antibody
    • Angiocidin antibody
    • Antisecretory factor 1 antibody
    • ASF antibody
    • DS5a antibody
    • MCB 1 antibody
    • MCB1 antibody
    • Multiubiquitin chain binding protein antibody
    • Multiubiquitin chain-binding protein antibody
    • OTTHUMP00000059963 antibody
    • Prosome macropain antibody
    • Proteasome (prosome macropain) 26S subunit non ATPase 4 antibody
    • Proteasome 26S non ATPase subunit 4 antibody
    • Proteasome 26S subunit non ATPase 4 antibody
    • PSMD 4 antibody
    • Psmd4 antibody
    • PSMD4_HUMAN antibody
    • pUB R5 antibody
    • pUBR5 antibody
    • Rpn 10 antibody
    • Rpn10 antibody
    • RPN10 homolog antibody
    • S5A antibody
    • S5a/antisecretory factor protein antibody
    see all

Anti-Proteasome 19S S5A antibody [AH1.1] 图像

  • Proteasome 19S S5A was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Mouse monoclonal to Proteasome 19S S5A and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab20239.

    Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/20,000 dilution.

    Band: 41kDa; Proteasome 19S S5A

  • ab20239 (1/200) staining Proteasome 19S S5A in Human retinal pigment epithelial (RPE) cells (green). Cells were fixed in Paraformaldehyde, permeabilised with 0.5% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue). Please refer to Abreview for further details.

    See Abreview

  • Overlay histogram showing HeLa cells stained with ab20239 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab20239, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-Proteasome 19S S5A antibody [AH1.1] (ab20239)参考文献

This product has been referenced in:
  • Liu YP  et al. Ciliopathy proteins regulate paracrine signaling by modulating proteasomal degradation of mediators. J Clin Invest 124:2059-70 (2014). WB . Read more (PubMed: 24691443) »
  • Kley RA  et al. Pathophysiology of protein aggregation and extended phenotyping in filaminopathy. Brain 135:2642-60 (2012). Read more (PubMed: 22961544) »

See all 5 Publications for this product

Product Wall

Thank you for contacting Abcam.

I have heard back from the lab and they have told me that with regard to your queries:

1) I believe that the antibody does cross react with mouse but unfortunately do not have any confirmed evidence t...

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I am very pleased to hear you would like to accept our offer and test these products in zebrafish. Each code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for zebrafish and include thi...

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Thank you for contacting Abcam. I am sorry about the delay in answering your questions as I have been waiting for the lab to respond.

According to the information that I have been able to obtain, ab20239 will not react with mouse S5A. We als...

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Thank you for contacting us.


This products immunogen is from the full S5A protein. We do not presently know the epitope that the antibody reacts against. I did run alignments for you against D.rerio and found84% identity between the h...

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Thank you for contacting us. That is correct, you should receive 91 ul.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEK293T)
Loading amount 5 µg
Specification HEK293T
Treatment siRNA
Gel Running Conditions Reduced Denaturing (4-12% invitrogen)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Tibor Pankotai

Verified customer

提交于 Sep 16 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (RPE-1)
Specification RPE-1
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton X100 in PBS
Username

Dr. Kirk McManus

Verified customer

提交于 Aug 14 2007

I have just received feedback from the source of ab20239 regarding your problem and have been informed that the information we received was wrong and that the antibody did not work very well in mouse samples, but worked better in human samples. My sin...

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I'm sorry to hear you are having a problem with ab20239. According to your order shipping details the antibody was shipped to you very rapidly with no delay, and we have not received any other complaints about this antibody. I am currently in touch...

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