Anti-Proteasome 19S S5A抗体(ab83344)

概述

  • 产品名称
    Anti-Proteasome 19S S5A抗体
    参阅全部 Proteasome 19S S5A 一抗
  • 描述
    兔多克隆抗体to Proteasome 19S S5A
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Saccharomyces cerevisiae, Caenorhabditis elegans, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to N terminal amino acids 1-50 (VLESTMVCVD NSEYMRNGDF LPTRLQAQQD AVNIVCHSKT RSNPENNVGL) of Human Proteasome 19S S5A (NP_002801).

  • 阳性对照
    • HepG2 cell lysate.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab83344 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 45, 55 kDa (predicted molecular weight: 40 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay 1/312500.

靶标

  • 功能
    Binds and presumably selects ubiquitin-conjugates for destruction. Displays selectivity for longer polyubiquitin chains. Modulates intestinal fluid secretion.
  • 序列相似性
    Belongs to the proteasome subunit S5A family.
    Contains 2 UIM (ubiquitin-interacting motif) repeats.
    Contains 1 VWFA domain.
  • 结构域
    The 2 UIM motifs are involved in the binding to a multi-ubiquitin chain in a cooperative way.
  • 翻译后修饰
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 26S protease subunit S5a antibody
    • 26S proteasome non ATPase regulatory subunit 4 antibody
    • 26S proteasome non-ATPase regulatory subunit 4 antibody
    • 26S proteasome regulatory subunit rpn10 antibody
    • 26S proteasome regulatory subunit S5A antibody
    • AF 1 antibody
    • AF antibody
    • AF1 antibody
    • Angiocidin antibody
    • Antisecretory factor 1 antibody
    • ASF antibody
    • DS5a antibody
    • MCB 1 antibody
    • MCB1 antibody
    • Multiubiquitin chain binding protein antibody
    • Multiubiquitin chain-binding protein antibody
    • OTTHUMP00000059963 antibody
    • Prosome macropain antibody
    • Proteasome (prosome macropain) 26S subunit non ATPase 4 antibody
    • Proteasome 26S non ATPase subunit 4 antibody
    • Proteasome 26S subunit non ATPase 4 antibody
    • PSMD 4 antibody
    • Psmd4 antibody
    • PSMD4_HUMAN antibody
    • pUB R5 antibody
    • pUBR5 antibody
    • Rpn 10 antibody
    • Rpn10 antibody
    • RPN10 homolog antibody
    • S5A antibody
    • S5a/antisecretory factor protein antibody
    see all

Anti-Proteasome 19S S5A antibody 图像

  • Anti-Proteasome 19S S5A antibody (ab83344) at 1 µg/ml + HepG2 cell lysate at 10 µg with skim milk/ PBS at 5 %

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 40 kDa
    Observed band size : 45,55 kDa (why is the actual band size different from the predicted?)

实验方案

Anti-Proteasome 19S S5A antibody (ab83344)参考文献

ab83344 has not yet been referenced specifically in any publications.

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