- 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- 存储溶液Preservative: None
Constituents: 2% Sucrose, PBS
Concentration information loading...
- 纯度Immunogen affinity purified
- Compatible Secondaries
- Positive Controls
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Detects a band of approximately 45, 55 kDa (predicted molecular weight: 40 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
||Use at an assay dependent concentration.
ELISA titre using peptide based assay 1/312500.
- 功能Binds and presumably selects ubiquitin-conjugates for destruction. Displays selectivity for longer polyubiquitin chains. Modulates intestinal fluid secretion.
- 序列相似性Belongs to the proteasome subunit S5A family.
Contains 2 UIM (ubiquitin-interacting motif) repeats.
Contains 1 VWFA domain.
- 结构域The 2 UIM motifs are involved in the binding to a multi-ubiquitin chain in a cooperative way.
- 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
Information by UniProt
- 26S protease subunit S5a antibody
- 26S proteasome non ATPase regulatory subunit 4 antibody
- 26S proteasome non-ATPase regulatory subunit 4 antibody
Anti-Proteasome 19S S5A antibody 图像
Western blot - Proteasome 19S S5A antibody (ab83344)
Anti-Proteasome 19S S5A antibody (ab83344) at 1 µg/ml + HepG2 cell lysate at 10 µg with skim milk/ PBS at 5 %
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 40 kDa
Observed band size : 45,55 kDa (why is the actual band size different from the predicted?)
Anti-Proteasome 19S S5A antibody (ab83344)参考文献
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"