Anti-Proteasome 19S S4抗体(ab3317)


  • 产品名称
    Anti-Proteasome 19S S4抗体
    参阅全部 Proteasome 19S S4 一抗
  • 描述
    兔多克隆抗体to Proteasome 19S S4
  • 特异性
    Detects proteasome 19S subunit S4.
  • 经测试应用
    适用于: IHC-P, ICC/IF, WB, IPmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Non human primates
  • 免疫原

    Other Immunogen Type corresponding to Proteasome 19S S4. Yeast proteasome 19S subunit S4.


  • 形式
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 纯度
    IgG fraction
  • 克隆
  • 同种型
  • 研究领域


Our Abpromise guarantee covers the use of ab3317 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/1000. PubMed: 18986984
WB 1/1000. Detects a band of approximately 56 kDa (predicted molecular weight: 49 kDa).

This antibody detects a 56 kDa protein representing proteasome 19S subunit S4 in HeLa cell lysate. This antibody detects, to a lesser extent, an ~35 kDa protein which could correspond to subunit degradation product.

IP Use at an assay dependent concentration.


Anti-Proteasome 19S S4 antibody 图像

  • Predicted band size : 49 kDa

    Western blot of proteasome 19S subunit S4 on HeLa cell lysate using ab3317.

  • ab3317 staining Proteasome 19S S4 in mock transfected rat primary neurons by Immunocytochemistry/ Immunofluorescence. Primary cultures of rat hippocampal neurons were prepared from E18 rats. Cells were fixed in 4% paraformaldehyde and incubated with primary antibody at 1/1000 dilution. The image show red staining with ab3317, nucleus was stained blue with Hoechst H33258 and right image show merged images. The green color seen in image is the GFP staining in similar experiment.

  • IHC image of ab3317 staining in human skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab3317, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Proteasome 19S S4 antibody (ab3317)参考文献

This product has been referenced in:
  • Zhou X  et al. HIV Replication in CD4+ T Lymphocytes in the Presence and Absence of Follicular Dendritic Cells: Inhibition of Replication Mediated by {alpha}-1-Antitrypsin through Altered I{kappa}B{alpha} Ubiquitination. J Immunol 186:3148-55 (2011). WB, IP . Read more (PubMed: 21263074) »
  • Rousseau E  et al. Misfolding of proteins with a polyglutamine expansion is facilitated by proteasomal chaperones. J Biol Chem 284:1917-29 (2009). WB, ICC/IF ; Human, Rat . Read more (PubMed: 18986984) »

See all 3 Publications for this product

Product Wall

Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 25°C
Aedes aegypti (Mosquito) Cell (c6/36)
Yes - .01 % TritonX100

Abcam user community

Verified customer

提交于 Nov 25 2014

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Thank you for your enquiry. I have been in touch with the source of this antibody and the originator has confirmed that this antibody was in fact raised using full length yeast proteasome 19S subunit S4 and was not a tagged version of the protein. ...

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Thank you for your enquiry. The originator of ab3317 has informed me that the concentration has not been determined and the expected band size in S.cerevisiae is not known to them. If you have any more questions, please contact us again.