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Our Abpromise guarantee covers the use of ab40879 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.|
|IHC-P||Use at an assay dependent concentration.|
|WB||1/10000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).|
|ICC/IF||Use at an assay dependent concentration. PubMed: 23350749|
ab40879 staining Prosurfactant Protein C in A549 cells from Human lung tissue by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were incubated with primary antibody (1/250). A FITC-conjugated anti-rabbit (1/200) was used as the secondary antibody. DAPI was used to detect the nucleus of the cells.
Immunohistochemical analysis of Pig lung tissue, labeling prosurfactant protein C with ab40879. Tissue fixed in 10% buffered formalin for 4 hours, and paraffin embedded. Anitgen retrieval performed in buffer at pH 9 at 75°C for 20 minutes. Blocked with PBS, treated with primary antibody, followed by secondary, and DAPI stained.
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