存放说明 Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
存储溶液 pH: 7.30 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA
Concentration information loading...
纯度 Immunogen affinity purified
纯化说明 ab116614 is purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.01 - 0.03 µg/ml. Predicted molecular weight: 48 kDa.
Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
序列相似性 Belongs to the histidine acid phosphatase family.
细胞定位 Secreted and Lysosome membrane. Predominantly localized in the plasma membrane but also detected in intracellular vesicles.
Information by UniProt
Acid phosphatase prostate antibody
Anti-Prostatic Acid Phosphatase antibody 图像
Western blot - Anti-Prostatic Acid Phosphatase antibody (ab116614)
Anti-Prostatic Acid Phosphatase antibody (ab116614) at 0.01 µg/ml + Human Prostate lysate at 35 µg developed using the ECL technique Predicted band size : 48 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostatic Acid Phosphatase antibody (ab116614)
ab116614 (3.8µg/ml) staining of paraffin embedded Human Prostate shows reticulate cytoplasm staining, more intens towards the lumen of the gland. Tissue Treatment: Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Anti-Prostatic Acid Phosphatase antibody (ab116614)参考文献
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"